Our three favorite parts containing the different versions of the KP6 toxin:
BBa_K2408020 - ADH1_KP6_proToxin: The toxin KP6 from the fungi U.maydis punched the outer membrane of B. bruxellensis and thus cause cell death. KP6 is translated as a protoxin and is then cleaved by protease into 2 subunits, alpha and beta. We have designed the part with the alpha MF secretion signal, ADH1 promoter, terminator and a histidine tag.
BBa_K2408021 - ADH1_KP6_alphaSubunit: The KP6 toxin’s alpha subunit, which is cleaved by protease from the KP6 protoxin (BBa_K2408020). This sequence also contains the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a 6 His tag.
BBa_K2408022 - ADH1_KP6_betaSubunit: The KP6 toxin’s beta subunit, which is cleaved by protease from the KP6 protoxin (BBa_K2408020). This sequence also contains the MF(ALPHA)1 secretion signal, ADH1 promoter, terminator and a 6 His tag.
Here are the parts we have created over our lab work. All our parts have been optimized to suit the yeast we worked with, Sacharomyces cerevisae.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2408001 | Coding | TAL | Yaakov Bulka | 1590 |
| BBa_K2408002 | Coding | 4CL | Yaakov Bulka | 1731 |
| BBa_K2408003 | Coding | STS_his_S.cerevisiae_Optimized | Yaakov Bulka | 1197 |
| MF_KP6_his | Coding | MF_KP6_his | Yaakov Bulka | 1173 |
| BBa_K2408005 | Coding | MF_Miraculin_his | Yaakov Bulka | 1176 |
| BBa_K2408010 | Regulatory | TDH3pro | Yaakov Bulka | 681 |
| BBa_K2408011 | Regulatory | PGK1pro | Yaakov Bulka | 781 |
| BBa_K2408012 | Regulatory | HXT7pro | Yaakov Bulka | 651 |
| BBa_K2408020 | Coding | ADH1_KP6_proToxin | Almog Angel, Michal Glikman | 1870 |
| BBa_K2408021 | Coding | ADH1_KP6_alphaSubunit | Almog Angel, Michal Glikman | 1189 |
| BBa_K2408022 | Coding | ADH1_KP6_betaSubunit | Almog Angel, Michal Glikman | 1195 |
| BBa_K2408023 | Coding | ADH1_Miraculin | Almog Angel, Michal Glikman | 1609 |
| BBa_K2408024 | Coding | HXT7_miraculin | Michal Glikman | 1233 |
