Team:XMU-China/More Chips

2017.igem.org/Team:XMU-China/More_Chips

Besides the microfluidic chip using in East-Wind, we have many other chips designed for freeze-dried bacteria checking and the part LuxAB. We also put the design drawing of the darkroom we use in the accessory below. You can get all the designs' CAD files here.

We design in the way of continuous integration, which means you will see the chips from the simplest chip in device, then plus LuxAB, then plus multichannel, then plus valve, then plus modularization.


-----* Chips *-----

I. Simplest chip in device





This design is what we use in the accessory. The size of sampling hole and tube has been tested many times. Making and using of this can be found here. There is a piece of semipermeable membrane between two breathe layers to avoid bacteria liquid leakage.



II. Plus LuxAB





This is the primary chip we design for LuxAB, which is a luciferase outputing chemiluminescence when adding substrate.

It has an inherent switch that you can't get the signal until a few seconds after add substrate. And it only needs one hour or less to get the signal after induction. So we design a set of cell and tube to add substrate to the reaction cell (the biggest cell); you can use this construction to control the time you open the switch (get signal).

This chip has four sampling channels and one detection channel. We have test the fluid flow in this chip, and we send this kind of chip to BIT.

The layers should be piled up with the order of 1 to 7 we mark in the picture. And the follow design can be assmbled in the same way.



III. Plus Multichannel



This design has four sampling channels and two detection channels. It enables us to have two channels of detection signal which will make it a less error equipment.



IV. Plus Valve



In this design, we make a valve in the chip so that you can control which sampling channel to add sample.



We also has this design of two sampling channels and four detection channels. You can control which detection channel to output.

In this section ,which of those two designs is more practical needs more experiments to test.



V. Plus Modularization



This chip should be assmbled into two parts of “1-2-3-4-4.4” and “4.6-5-6”, which we name as partA and partB. You can use partB with different partA in which we have added different engineered bacteria.

-----* Darkroom *-----

I. Primary Darkroom





We design this darkroom to make a steady optical environment for our accessory to work. You can see how to assumble and use it in our wiki.



II. Darkroom Drawer





When you use this daro room, you can put your chip in different layer for your special need.