HPLC Conditions
Eluent A
2.5 : 97.5 % (v/v) acetic acid: H2O, pH 3.8
*pH was adjusted by addition of 1 mol L-1 KOH
Eluent B
80 : 20 % (v/v) acetonitrile: H2O
Gradient Elution (A>B)
Time Scale(min) @ FR (1ml/min)
Analysis
Initial Standard Run of Tryptophan
MW of compounds:
Tryptophan MM: 204.225 g/mol
Indole-3-pyruvic acid MM: 203.197 g/mol
Indole-3-acetaldehyde MM: 159.188 g/mol
Tryptophol MM: 161.20 g/mol
Tryptophan and Indole-3-pyruvic acid
Tryptophan and indole-3-pyruvic acid are too close in MM that unintended reactions during the run would make it difficult to differentiate the two.
