Toolbox modules assembly
How we constructed our circuits
core breakthroughs
Construction of standard interchangeable parts for genome engineering based on CRISPR-Cas9 machinery;
Engineering of an optimized sgRNA (both crRNA design AND tracrRNA structure) to amplify editing efficiency;
CRISPeasy-out method (crOUT)
core breakthroughs:
Characterization of the Cas9 device + optimized sgRNA (BBa_K2457006) in E. coli K12 MG1655 cells by tracking the impact on the cellular system (i.e., growth ratio);
Setting up a reliable data sheet of Cas9 activation machinery in a range of conditions to further expand its uses by SynBio and iGEM community;
