Composite Parts
This year, we CSMU_NCHU_Taiwan, brings you 3 composite parts.
Composite Parts
FOR ANTIDOTE
T7 promoter + Thioredoxin-FGD fusion protein (BBa_K2382005)
This part contains a T7 promoter and Thioredoxin-FGD fusion protein, and terminators are
included. By ligating these two different parts as a fusion protein, it is supposed to raise the
solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).
T7 promoter + Thioredoxin-MSMEG_5998 fusion protein (BBa_K2382006)
This part contains a T7 promoter and Thioredoxin-MSMEG_5998 fusion protein, and terminators
are included. By ligating these two different parts as a fusion protein, it works better than
MSMEG_5998 alone.
FOR TEST STRIP
Anti-aflatoxin scFv fusion protein construction DNA sequence (BBa_K2382010)
By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034)
and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing
in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future
iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator,
and make their own test strip!