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RESULTS
- Module 1: An optogenetic toggle switch circuit capable of tuning the expression of a desired protein depending on the wavelength of the light stimuli was developed.
- Red activation of the optogenetic toggle switch was characterized.
- Luciferase dynamic in optogenetic circuit was analysed.
- Root-aerial part transport by viral autoreplicative vectors was demonstrated.
- Module 2: A Color Code System triggered by external inducible stimulus together with stress-signals was achieved in order to flag any change in their status accelerating corrective measures.
- PhiC31 was characterized.
- Metabolic load vs unproductive complex theory hypothesis were assessed.
- Recombination Directionality Factor (RDF) action was demonstrated.
- RDF was characterized.
- Stress-inducible promoter feasibility was demonstrated.
- Color code protein expression through a viral vector strategy was achieved.
- Chromoproteins expression in Nicotiana benthamiana was tested.
- Module 3: A hardware device (ChatterBox) was engineered in order to provide a SynBio-based solution to improve agriculture sustainability.
- Module 4: PlantLabCo, an open-access platform aiming to unify Plant SynBio researchers work, was developed.