The goal of this project is to engineer a biological circuit utilizing multiple E. coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect arsenic. Once this construct is designed and constructed the circuit will be tested to demonstrate its viability using a cell free lateral flow paper based system designed to detect arsenic.
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Synthetic Biology is the shift from understanding and modifying biology to treating biology as an engineering discipline where basic building blocks are assembled from the ground up into larger engineering systems.
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Develop tools to work at increasing levels of abstraction and enable facile manufacturing of designs
Goal #2
Leverage expertise from established engineering disciplines
Goal #3
Ultimately, forward engineer novel solutions to diverse problems
RESOURCES
Throughout this project we worked closely with several local non-profits in the Tacoma urban area. The goal of the project was to design a successful biological circuit utilizing e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic+.
After the circuit was designed and constructed the next step was to demonstrate the circuit as viable in a cell of Arsenic. To accomplish this goal we worked with Urban Waters, Readiness Acceleration and Innovation Networking (RAIN),and Charles Wright Academy.
By utilizing community involvement we hope to both increase pre-collegiate interest in STEM related fields as well as increase the communal understanding of the environment and provide a inexpensive and effective tool for the detection of potentially toxic metals.
