All parts were codon-optimized for expression in E. coli Nissle with Geneious and modified to remove forbidden restriction sites.
BBa_K2500008: PConst_RBSeng_TlpA
Design notes: A RBS library was created using the Red Libs algorithm to find variants translating more TlpA RNA in order to create a thermoswitch tight enough to repress the premature induction of cell lysis by protein E.