Team:BOKU-Vienna/Notebookdata

Monday, July 3rd.

• Team discussion and overview:
• Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
• Planning of the procedures including primer design
• Preparing the growth media and autoclaving [LINK ZUM PROTOKOLL]
• LB medium preparation (4L): Mina, Michael
• Autoclaving: Mina, Rebecca

Tuesday, July 4th.

• Planning and fundraising:
• Christina, Felicia, Josef, Lisa, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
• Preparation of growth media: [LINK ZUM PROTOKOLL]
• 20 plates LB medium + chloramphenicol: Mina, Lisa, Mathias
• 2 flasks LB agar: Mina, Lisa, Mathias

Wednesday, July 5th.

• Planning and fundraising:
• Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
• Aliquoting 1mL LB into Eppendorf tubes
• Lisa, Josef
• Transferring Backbones onto agar plates:
• Julian
• Interlab challenge - transformation: According to protocol (Preparation of chemically competent E. Coli with RbCl and transformation [HIER LINK]):
Lisa, Mathias, Michael, Mina, Rebecca
• 8 transformations - from Kit Plate 6 InterLab Part Locations
• 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
• stored at 35°C

Thursday, July 6th.

• Team discussion, planning and fundraising
• Counting colonies of Transformants for the Interlab Challenge: [HIER LINK ZU ERGEBNISSEN]
• Colonies 200µL: 77, 20µL: 26, 100µL: 43
• Miniprep of 8 plasmids for Golden Gate Standard - according to protocol [HIER LINK]
• Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
• Dilution of plasmids: Lisa [LINK ZU DILUTION SHEET]

Friday, July 7th.

• Team discussion, Planning and Fundraising
• Interlab Challenge: Fluorescein fluorescence standard curve
• Christina