Team:Munich/Demonstrate

-_- Project Description Design Demonstrate Measurement Applied Design Final Results Achievements Wetlab Protocols Notebook Safety Parts Improved Part Part Collection InterLab Model/Software Model Software Collaboration Hardware Detector Quake Valve Paperstrips Sample Processing Human Practices Silver Gold Engagement Collaborations Team Team members Sponsors Attributions Gallery

Demonstrate With our modular approach, we prototype all units of our diagnostic device, and integrate them into a customizable platform. We use cell-free synthetic biology to distinguish between pathogens with our universal detection cascade. Sensitive and Rapid Robust and Universal Portable and low-cost We chose to combine heat lysis and isothermal amplification (RPA) to extract our target RNA from patient samples Demonstrated functionality of Lbu and Lwa Cas13a. Modelled the detection limit of our circuit and confirmed it experimentally (~10 nM RNA). Detected pathogen RNA sequence from in vitro and in vivo sources. Differentiated viral sequences from bacterial sequences. Used RNase Alert and Spinach aptamer read-out circuits. Used gold nanoparticles to detect general RNase activity. Constructed a functional fluorescence detector with high sensitivity and low production cost. Detected RNA in bulk, on paper, and from lyophilized Cas13a. Amplified target with RPA and transcription on paper. Improved the biobrick BBa_K1319008 by adding a 6x His-tag and provided Cas13a Lwa as three different composite biobricks. Characterized the GFP degradation tags and sent them as a part collection.