Poster printed, presentation...needs a bit more practicing
Characterisation/Contribution
We have participated in the Interlab Study and we have sent our results to iGEM HQ.
Attribution
Numerous individuals and companies have shaped our project from start to finish, we have thanked each one of these on our attributions page.
Validated Part
We proved that the T7_FimH_225sfGFP part worked as expected, because after induction, the flow cytometer and plate reader showed a significant amount of fluorescence compared to the wild type.
Collaboration
We collaborated with both the Newcastle iGEM team , where we obtained data for them using our FACS machine whilst in return of they repeated an experiment of ours, and Cardiff’s iGEM team who repeated and extended an experiment of us.
Human Practices
Throughout the project we implemented the AREA framework to ensure we were continuously conducting responsible research.
We improved the previous part pRha - fimH - SpyTag_225 - HisTag_225 from the Harvard 2015 iGEM team. We have removed the Spy Tag to make and moved the His tag from the 225th residue, making pRha_FimH_1His. We have further characterised the part by doing a Western Blot and a TEM with Immunogold images.
Model your project
We created and designed a model to inform the future industrial implementation of our project which has been incorporated into a friendly user interface and showcased on our wiki, click here.