Team:Aix-Marseille/SN
SuperNova activity
We wanted to test the activity of [http://parts.igem.org/Part:BBa_K1491018 Supernova] on the colonies, so we made a protocol where we illuminated petri dish with a 16,5 Candela 595nm Diode (lumex ssl-lx5093syc/g) for 2 hours.
With the following protocol :
- Transform 100µL TG1 with Eprc - SN (psb1C3 plasmid, 5µL)
- To get an adequate number of colonies :
- Into 2mL LB add 2µL of 2 DO TG1
- Pipet up and down
- From the first dilution, take 8µL and dilute it into 1mL LB
- Spin at 5000rpm for 10min
- Remove 850µL supernatant
- Resuspend pellet (even if you can't see it)
- Spread remaining 150µL on petri dish
- Immediatly put on 590nm light / put control in dark
- After two hours, incubate overnight
- Count colonies
| Exposed | Not exposed | |
| Supernova | 1496 | 3672 |
| RFP | 3545 | 3638 |
The with a RFP plasmid as a control we show there's no decrease.
With this experiment, we can conclude that supernova allows us to divide the number of colonies by 2,45.
