Team:BOKU-Vienna/Notebookdata
Monday, July 3rd.
- Team discussion and overview:
- Christina, Felicia, Josef, Lisa, Magdalena, Mathias, Melanie, Michael, Mina, Rebecca, Valentin, Julian
- Planning of the procedures including primer design
- Preparing the growth media and autoclaving
- LB medium preparation (4L): Mina, Michael
- Autoclaving: Mina, Rebecca
Tuesday, July 4th.
Wednesday, July 5th.
- Planning and fundraising:
- Christina, Felicia, Josef, Lisa, Magdalena, Martin, Mathias, Melanie, Michael, Mina, Rebecca, Julian
- Aliquoting 1mL LB into Eppendorf tubes
- Lisa, Josef
- Transferring Backbones onto agar plates:
- Julian
- Interlab challenge - transformation: According to protocol:
- 8 transformations - from Kit Plate 6 InterLab
- 8 plasmids plated onto agar medium: Positive control (50µL, 200µL), Negative Control (50µL, 200µL), Transformant (20µL, 100µL, 200µL), Test device 1-6 (50µL, 200µL)
- stored at 35°C
- Lisa, Mathias, Michael, Mina, Rebecca
Thursday, July 6th.
- Team discussion, planning and fundraising
- Counting colonies of Transformants for the Interlab Challenge:
- Colonies 200µL: 77, 20µL: 26, 100µL: 43
- Miniprep of 8 plasmids for Golden Gate Standard - according to protocol
- Magdalena (1), Melanie (2), Valentin (3), Rebeccca (4), Christina (5), Martin (6), Mina (7), Lisa (8)
- Dilution of plasmids for Golden Gate: Lisa
Dilution sheet for Golden Gate Assembly
| Sample | Volume of AD - RO Water [µL] | Volume of plasmid/fragment [µL] |
|---|---|---|
| BB1-C-23-Spy-Cas9(NLS)-49 | 71,6 | 28,4 |
| BB1-T-34-sce-cyc-50 | 83,0 | 17,0 |
| BB1-P-12-syr-pNS-J23101-25 | 85,1 | 14,9 |
| BB1-P-12-sce-tdh3-45 | 82,7 | 17,3 |
| BB1-T-34-eco-B1001-29 | 80,4 | 19,6 |
| BB1-P-12-syn-pm-J23105-23 | 83,9 | 16,1 |
| BB1-C-23-syn-GFP-30 | 82,9 | 17,1 |
| BB1-P-12-sce-tef1-46 | 78,0 | 22,0 |
Friday, July 7th.
- Team discussion, Planning and Fundraising
- Interlab Challenge: Fluorescein fluorescence standard curve
- Christina
