Team:Cadets2Vets/Design

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Design - USE THIS ONE!!!

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DESIGN



Our circuit was designed to utilize specific parts so that when the E-coli cells containing this gene circuit are exposed to arsenic, the Green Florescent Protein (GFP) is produced making the ticket glow under a blue light.





THE FOUNDATION OF OUR CIRCUIT



pHASE 1



  • Test efficacy of the circuit in vivo
  • Grow transformed e.coli with plasma circuit in Arsenic laced media
  • Serial dilution of arsenic and arsenic to find the lowest activation amount of the circuit in vivo


pHASE 2



  • Test the efficacy of the plasma circuit in S.I.T.U.
  • Perform above experiments with prepared plasma circuit
  • Titrate both circuit and trigger to find optimal mixture
  • Standardize reactions
  • Test on paper matrix


pHASE 3



  • Test efficacy of freeze drying circuit on paper for distribution and storage purposes.


Design of our circuit



BY: Ryan Brisbin





Thought Process





Testing our Product




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2304 Jefferson Ave, Tacoma, WA 98402