Team:Glasgow
Campylobacter
Our project idea is to develop a biosensor to detect the presence of the bacteria Campylobacter. This sensor will utilise the rare sugar xylulose, which is found in the polysaccharide capsule of campylobacter and is released when the bacteria is run through an acidic solution. By exploiting the mannitol operon that is present in the bacteria Pseudomonas fluorescens and expressing this in our chassis organism, Escherichia Coli, we will produce a biosensor that will express the reporter molecule Green Fluorescent Protein (GFP) when xylulose interacts with the repressor molecule of the mannitol operon. Additional sub-projects will include; investigating the quorum sensing mechanisms in campylobacter to increase the specificity of our sensor, developing hardware to produce a functioning biosensor and investigating the legal and ethical issues associated with our project.
Explore ItIGEM 2017
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