Charaterization
Fluorescent protein is an important marker for qualitative analysis in validation test of toehold switch which detects the presence of trigger (viral) RNA.The presence of trigger RNA is reported by fold change of fluorescent protein expression compared with switch only by fluorescent assays and rely on the ability of fluorescent protein. More often, body fluid of different pH from vary organs (Figure 1) extracted as sample may disturb the expression of fluorescent protein due to pH-dependent protein folding and conformtaion.
Key achievement:
- Charaterized pH stability of fluorescnet protein in iGEM registry.