Team:Hong Kong-CUHK/Notebook
Home
Project
Description
Experiments
Results
Parts
InterLab
Modelling
Software
Notebook
Notebook
Protocols
Human Practices
Integrated Human Practices
Engagement and Education
Prototype
Collaboration
Team
About Us
Attributions
April 2016
Settlement of lab duties and position.
Lab safety Training.
Started regular meetings every week.
May 2016
Recruitment of ideas and prepare preliminary data for selection.
Project related to pancreatic cancer diagnosis by Toehold Switch is selected
August 2016
Cloning of switches and itscorresponding triggers into pSB1A2 and pSB1 with significant “ON/OFF”ratio in vivo.
September 2016
Identified problems to design Toehold Switch manually and outline programme functions for semi-automatic toehold switch design.
Modified the switch by using new chromoprotein (amajLime and amilCP) by traditional cloning method to produce intuitive readout.
October 2016
Reviewing the impact and necessity of technology in nowadays society, we decided to use Toehold Switch to subtype Influenza A.
November 2016
Brainstorming and literature reviewing on how to maximize the“ON/OFF” ratio of Toehold switch.
Constructed programs which embedded with Influenza A sequence database and Vienna RNA package.
December 2016
Failed to test the system in cell free system due to insufficient materials and resources.
T7-rna-polymerase amplification system is cloning to yield higher “ON/OFF”
Requested for sponsorship.
Skyped the Pakistan iGEM team to explore the potential of collaboration.
Continued fundraising.
