Experiments Performed:
Validate the use of LC 480 II utilizing T7 constitutive GFP construct.
QC/ QA of Ars. 1.1 plasmid cel
Culture of Ars 1.1 transformed E. coli were exposed to 25 uM Aresnate + Aresnite at 37 C in the LC 480 II for 24 hours
Why We Performed them:
We need a way to properly quantify GFP production and QC/QA the plasmid
Results:
Validating the use of LC 480 II utilizing T7 constitutive GFP construct
The LC 480 II was successfully able to monitor the increase GFP over the incubation time
QC/QA the plasmid
Circularized plasmid gels proved inconclusive however M13 amplification did provide strong indication of proper fragment. The fragment size is 1461 bp, with the second bright band in the ladder being equal to 1650 bp. All midipreps exhibit a band slight below the 1625 bp band of the ladder, which is evident of proper fragment size.
