Team:Harvard/InterLab
InterLab
Overview
Difficulty in taking reliable and reproducible measurements remains a key obstacle in establishing synthetic biology as an engineering discipline. The InterLab Study is part of the Measurement Committee’s continuing effort to develop a standard measurement procedure for green fluorescent protein (GFP). Despite being one of the most commonly used markers in synthetic biology, labs often resort to making relative comparisons, which makes it difficult for labs to share and data and/or constructs.
For the fourth installment of the InterLab, iGEM hopes to establish a GFP measurement protocol that can be used to produce comparable GFP measurements on different plate readers. In addition, iGEM teams from around the world have tested RBS devices (BCDs) intended to increase the precision and reliability of gene expression. The parts used include six test devices (BBa_J364000, BBa_J364001, BBa_J364002, BBa_J364003, BBa_J364004, BBa_J364005), as well as a positive (BBa_I20270) and negative (BBa_R0040) control. All parts are located in the pSB1C3 plasmid and carry chloramphenicol resistance.
Methods and Materials
- 100 uL of LUDOX-S40 from the InterLab Measurement Kit added into wells A1, B1, C1, and D1
- 100 uL of dH2O added to wells A2, B2, C2, D2
- Absorbance at 600 nm taken for all samples in setup described above
- Data used to obtain correction factor
