Team:SDU CHINA
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Abstract
The incidence of cancer is increasing in modern society. In all cancer patients, P53 mutations account for nearly 50%. If the mutant P53 can be replaced with wild P53 using gene therapy method, it will be the best theropy for those patients. In our project, we are using CRISPR technique to cut the mutant p53 in tumor cells. In the pro cess of genome repair, wild p53 will be integrated into genome via the principle of homologous recombination, restructuring the genome of cancer cells so as to realize the replacement of mutant p53. The characteristics of our project is that after the mutant p53 is replaced, the transfected plasmids will commit suicide. Even if the plasmids are transfected into the nontumorous cells, plasmids will also cut and degrade themselves. So it will not pose a threat to normal cells, achieving the safety of gene therapy.
