Difference between revisions of "Team:Kingsborough NY/BioBrick"

(Created page with "{{Template:Kingsborough_NY}} Replace this with text describing the construction of our BioBricks (3.1 and 3.2) - in other words, the colony PCR and sequencing Data")
 
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Replace this with text describing the construction of our BioBricks (3.1 and 3.2) - in other words, the colony PCR and sequencing Data
 
Replace this with text describing the construction of our BioBricks (3.1 and 3.2) - in other words, the colony PCR and sequencing Data
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== PCR to Generate Fragments for Gibson Assembly ==
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Text here about the PCR reactions to create the fragments for Gibson Assembly
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 +
[[File:T--Kingsborough_NY--gibson_fragment_pcr1.jpg|800px|center]]
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<br clear=all>
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== Colony PCR to Verify Clone is Correct ==
 +
 +
Text here about the PCR reactions to create the fragments for Gibson Assembly
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 +
[[T--Kingsborough_NY--colony_pcr1.jpg|800px|center]]
 +
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<br clear=all>
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== Sequencing Verification ==
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Text here about the PCR reactions to create the fragments for Gibson Assembly
 +
 +
Example sequencing results for construct K2268004
 +
 +
> VF2 Primer result, reverse complement
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GGGNACGTTACGTTGCGCATTTAACTATTAGATGCGTCGAATCACGAGGCAGGAATTTCAGATAAAAAAAATCCTTAGCT
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TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTG
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ATAATGGTTGCGCTAGCAAAGAGGAGAAAGCTTATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACTCGTGAC
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TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGC
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CCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAAT
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> 3.2_LinkR Primer result, reverse complement.
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ATCACCAGCTCCCGTCTTTCATTGCATACGAAATTCCGGAGAGCATTCATCAGGCGGGCA
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AGAAATGTGAATAAAGGCCGAATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAA
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GGCCGTAATATCCAACTGAACGGTCTGGTTATAGGTACATTGAGCACTGACTGAAATGCC
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CTCAAAATGTTCTTTACGATGCCATTGGGATATATCACCGGTGGTATATCCAGTGATTTT
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TTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGG
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TAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG
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TGCCACCTGACGTCTAAGAACCCATTATTATCGTGACATTAACCTATAAAAATAGGCGTA
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TCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGA
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ATTCGCGGCCGCTTCTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTGAT
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AATGGTTGCGCTAGCAAAGAGGAGAAAGCTTATGACCATGATTACGGATTCACTGGCCGT
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CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGC
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ACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCA
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ACAGAGGGCAGCTTGAATGGCGAATGGGCTTTGCCTGGTTTTTCGAATTCTTCCGCAACT
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TACTGCGTAGCGNA
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> 3.2_LinkF Primer result
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NTCCATGATAAGTACTCGCACTTTCTCAGCAGTCATCAGTTCGCCGCGCCAAACGTCTCTTCAGGCCACTGACTAGCGAT
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AACTTTCCCCACAACGGAACAACTCTCATTGCATGGGATCATTGGGTACTGTGGGTTTAGTGGTTGTAAAAACACCTGAC
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CGCTATCCCTGATCAGTTTCTTGAAGGTAAACTCATCACCCCCAAGTCTGGCTATGCAGAAATCACCTGGCTCAACAGCC
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TGCTCAGGGTCAACGAGAATTAACATTCCGTCAGGAAAGCTTGGCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCTTC
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AACCTCAAGCCAGAATGCAGAATCACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCCGCATCACCTTTGGTAAAGGTTC
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TAAGCTAAGGTGAGAACATCCCTGCCTGAACATGAGAAAAAACAGGGTACTCATACTCACTTCTAAGTGACGGCTGCATA
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CTAACCGCTTCATACATCTCGTAGATTTCTCTGGCGATTGAAGGGCTAAATTCTTCAACGCTAACTTTGAGAATTTTTGC
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AAGCAATGCGGCGTTATAAGCATTTAATGCATTGATGCCATTAAATAAAGCACCAACGCCTGACTGCCCCATCCCCATCT
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TGTCTGCGACAGATTCCTGGGATAAGCCAAGTTCATTTTTCTTTTTTTCATAAATTGCTTTAAGGCGACGTGCGTCCTCA
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AGCTGCTCTTGTGTTAATGGTTTCTTTTTTGTGCTCATAAGCTTTCTTCCTCTTTGCTAGCTGTGCTCAGTATCTTGTTA
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TCCGCTCACAATTTACTAGTAGCGGCCGCTGCAGGCCCGGCAAAAAAGGCAAGGGGTCACCACCCTGCCCTTTTTCTTTA
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AAACCGAAAAGATTACTTCGCGTTATGCAGGGTTCCTCGCTCACTGACTCGCTGCGCTCGGGCGTCGGCTGCGGCGGAGC
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GGATCAGCTCACTCAAGCGGTAATACGGTATCACAGAATCAGGGATACCGCAGAAGAACATGTGAGCAAAGGCAGCAAAG
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GCAGGACTCGTAAAAGCCGCGTGCTGGCGATTCACAAGGCTCGCCCCCTGACGGCACTACAAAATCGACGCTCTAGGCAG
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AGGTGCGAACCGCACGGACATAAAGGATCCAGC
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> VR Primer result, reverse complement
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ATGATTCGGATCCCTGGCCGTGTTTACACGTTCGGAACTGGGAAACCCTGCGTACCAACT
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AATCCCTTGCAGCACATCCCTTTCGCAGCTGCGTAATAGCGAAGAGGCCGCCCGATCGCC
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CTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCGCTTGCCTGGTTTCCGGCACCAGAA
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GCGGTGCCGAAAGCTGGCTGGAGTAATATTATTACGCCACCAGCGCATAGTTTTCATCGT
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TCGCGCGCAAACGTCTCTTCAGGCACTGACTAGCGATAACTTTCCCCACAACGGAACACC
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TCTCATTGCATGGGATCATTGGGTACTGTGGGTTTAGTGGTTGTAAAAACACCTGACCGC
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TATCCCTGATCAGTTTCTTGAAGGTAAACTCATCACCCCCAAGTCTGGCTATGCAGAAAT
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CACCTGGCTCAACAGCCTGCTCAGGGTCAACGAGAATTAACATTCCGTCAGGAAAGCTTG
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GCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCTTCAACCTCAAGCCAGAATGCAGAAT
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CACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCCGCATCACCTTTGGTAAAGGTTCTAA
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GCTAAGGTGAGAACATCCCTGCCTGAACATGAGAAAAAACAGGGTACTCATACTCACTTC
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TAAGTGACGGCTGCATACTAACCGCTTCATACATCTCGTAGATTTCTCTGGCGATTGAAG
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GGCTAAATTCTTCAACGCTAACTTTGAGAATTTTTGCAAGCAATGCGGCGTTATAAGCAT
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TTAATGCATTGATGCCATTAAATAAAGCACCAACGCCTGACTGCCCCATCCCCATCTTGT
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CTGCGACAGATTCCTGGGATAAGCCAAGTTCATTTTTCTTTTTTTCATAAATTGCTTTAA
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GGCGACGTGCGTCCTCAAGCTGCTCTTGTGTTAATGGTTTCTTTTTTGTGCTCATAAGCG
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TTAGTATAACATTGAAGAGCTATTCGCATCACACGGATCTCCGAGCGCATTGAGCCATTG
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AACGAGGAATCGGAAGAGCGCCCATTAATCAATCGCCCCTCGCGCGGGGAGAGGCGGTTT
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GCGTATTGGGCGCTCTGCCCCTCACTCGCTCCCTCTGGAAACTGCGGCAGTACGTTGCGC
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GGAATGTAAGG
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<br clear=all>

Revision as of 02:44, 1 November 2017

Kingsborough Community College || City Unversity of New York || 2017 iGEM Team

Computer Hope
TEAM
DESIGN
Part Design
Methods
Model
RESULTS
Seawater Survival
Bootcamp
RFP Characterization
BioBrick Construction
LacZ-alpha Controls
Demonstrated Results
SAFETY
HUMAN PRACTICES
Human Practices
Integrated and Gold
Public Engagement
JUDGING FORM

Replace this with text describing the construction of our BioBricks (3.1 and 3.2) - in other words, the colony PCR and sequencing Data

PCR to Generate Fragments for Gibson Assembly

Text here about the PCR reactions to create the fragments for Gibson Assembly

T--Kingsborough NY--gibson fragment pcr1.jpg


Colony PCR to Verify Clone is Correct

Text here about the PCR reactions to create the fragments for Gibson Assembly

800px|center


Sequencing Verification

Text here about the PCR reactions to create the fragments for Gibson Assembly

Example sequencing results for construct K2268004

> VF2 Primer result, reverse complement GGGNACGTTACGTTGCGCATTTAACTATTAGATGCGTCGAATCACGAGGCAGGAATTTCAGATAAAAAAAATCCTTAGCT TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTG ATAATGGTTGCGCTAGCAAAGAGGAGAAAGCTTATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACTCGTGAC TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGC CCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAAT


> 3.2_LinkR Primer result, reverse complement. ATCACCAGCTCCCGTCTTTCATTGCATACGAAATTCCGGAGAGCATTCATCAGGCGGGCA AGAAATGTGAATAAAGGCCGAATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAA GGCCGTAATATCCAACTGAACGGTCTGGTTATAGGTACATTGAGCACTGACTGAAATGCC CTCAAAATGTTCTTTACGATGCCATTGGGATATATCACCGGTGGTATATCCAGTGATTTT TTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGG TAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG TGCCACCTGACGTCTAAGAACCCATTATTATCGTGACATTAACCTATAAAAATAGGCGTA TCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGA ATTCGCGGCCGCTTCTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTGAT AATGGTTGCGCTAGCAAAGAGGAGAAAGCTTATGACCATGATTACGGATTCACTGGCCGT CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGC ACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCA ACAGAGGGCAGCTTGAATGGCGAATGGGCTTTGCCTGGTTTTTCGAATTCTTCCGCAACT TACTGCGTAGCGNA

> 3.2_LinkF Primer result NTCCATGATAAGTACTCGCACTTTCTCAGCAGTCATCAGTTCGCCGCGCCAAACGTCTCTTCAGGCCACTGACTAGCGAT AACTTTCCCCACAACGGAACAACTCTCATTGCATGGGATCATTGGGTACTGTGGGTTTAGTGGTTGTAAAAACACCTGAC CGCTATCCCTGATCAGTTTCTTGAAGGTAAACTCATCACCCCCAAGTCTGGCTATGCAGAAATCACCTGGCTCAACAGCC TGCTCAGGGTCAACGAGAATTAACATTCCGTCAGGAAAGCTTGGCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCTTC AACCTCAAGCCAGAATGCAGAATCACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCCGCATCACCTTTGGTAAAGGTTC TAAGCTAAGGTGAGAACATCCCTGCCTGAACATGAGAAAAAACAGGGTACTCATACTCACTTCTAAGTGACGGCTGCATA CTAACCGCTTCATACATCTCGTAGATTTCTCTGGCGATTGAAGGGCTAAATTCTTCAACGCTAACTTTGAGAATTTTTGC AAGCAATGCGGCGTTATAAGCATTTAATGCATTGATGCCATTAAATAAAGCACCAACGCCTGACTGCCCCATCCCCATCT TGTCTGCGACAGATTCCTGGGATAAGCCAAGTTCATTTTTCTTTTTTTCATAAATTGCTTTAAGGCGACGTGCGTCCTCA AGCTGCTCTTGTGTTAATGGTTTCTTTTTTGTGCTCATAAGCTTTCTTCCTCTTTGCTAGCTGTGCTCAGTATCTTGTTA TCCGCTCACAATTTACTAGTAGCGGCCGCTGCAGGCCCGGCAAAAAAGGCAAGGGGTCACCACCCTGCCCTTTTTCTTTA AAACCGAAAAGATTACTTCGCGTTATGCAGGGTTCCTCGCTCACTGACTCGCTGCGCTCGGGCGTCGGCTGCGGCGGAGC GGATCAGCTCACTCAAGCGGTAATACGGTATCACAGAATCAGGGATACCGCAGAAGAACATGTGAGCAAAGGCAGCAAAG GCAGGACTCGTAAAAGCCGCGTGCTGGCGATTCACAAGGCTCGCCCCCTGACGGCACTACAAAATCGACGCTCTAGGCAG AGGTGCGAACCGCACGGACATAAAGGATCCAGC


> VR Primer result, reverse complement ATGATTCGGATCCCTGGCCGTGTTTACACGTTCGGAACTGGGAAACCCTGCGTACCAACT AATCCCTTGCAGCACATCCCTTTCGCAGCTGCGTAATAGCGAAGAGGCCGCCCGATCGCC CTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCGCTTGCCTGGTTTCCGGCACCAGAA GCGGTGCCGAAAGCTGGCTGGAGTAATATTATTACGCCACCAGCGCATAGTTTTCATCGT TCGCGCGCAAACGTCTCTTCAGGCACTGACTAGCGATAACTTTCCCCACAACGGAACACC TCTCATTGCATGGGATCATTGGGTACTGTGGGTTTAGTGGTTGTAAAAACACCTGACCGC TATCCCTGATCAGTTTCTTGAAGGTAAACTCATCACCCCCAAGTCTGGCTATGCAGAAAT CACCTGGCTCAACAGCCTGCTCAGGGTCAACGAGAATTAACATTCCGTCAGGAAAGCTTG GCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCTTCAACCTCAAGCCAGAATGCAGAAT CACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCCGCATCACCTTTGGTAAAGGTTCTAA GCTAAGGTGAGAACATCCCTGCCTGAACATGAGAAAAAACAGGGTACTCATACTCACTTC TAAGTGACGGCTGCATACTAACCGCTTCATACATCTCGTAGATTTCTCTGGCGATTGAAG GGCTAAATTCTTCAACGCTAACTTTGAGAATTTTTGCAAGCAATGCGGCGTTATAAGCAT TTAATGCATTGATGCCATTAAATAAAGCACCAACGCCTGACTGCCCCATCCCCATCTTGT CTGCGACAGATTCCTGGGATAAGCCAAGTTCATTTTTCTTTTTTTCATAAATTGCTTTAA GGCGACGTGCGTCCTCAAGCTGCTCTTGTGTTAATGGTTTCTTTTTTGTGCTCATAAGCG TTAGTATAACATTGAAGAGCTATTCGCATCACACGGATCTCCGAGCGCATTGAGCCATTG AACGAGGAATCGGAAGAGCGCCCATTAATCAATCGCCCCTCGCGCGGGGAGAGGCGGTTT GCGTATTGGGCGCTCTGCCCCTCACTCGCTCCCTCTGGAAACTGCGGCAGTACGTTGCGC GGAATGTAAGG