Difference between revisions of "Team:Kingsborough NY/RFP"
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As part of our experiments to determine if genetically modified E. coli can grow and thrive in salt water, we plated dilutions of E. coli exposed to different media on agar to obtain CFU/mL counts. We plated them not only on LB + chloramphenicol, but also LB with 3% NaCl (mimicking seawater conditions) to recover any other microorganisms that might be present in the local water mixtures we used. Surprisingly, the E. coli that grew on these plates seemed to not only grow slower, but express RFP to a much lower extent (a decreased fluorescence that was not recovered when given a longer incubation time). | As part of our experiments to determine if genetically modified E. coli can grow and thrive in salt water, we plated dilutions of E. coli exposed to different media on agar to obtain CFU/mL counts. We plated them not only on LB + chloramphenicol, but also LB with 3% NaCl (mimicking seawater conditions) to recover any other microorganisms that might be present in the local water mixtures we used. Surprisingly, the E. coli that grew on these plates seemed to not only grow slower, but express RFP to a much lower extent (a decreased fluorescence that was not recovered when given a longer incubation time). | ||
| − | [[File:T--Kingsborough_NY--seawater_RFP_LB_cm.jpg|400x300px| | + | [[File:T--Kingsborough_NY--seawater_RFP_LB_cm.jpg|400x300px|center]] [[File:T--Kingsborough_NY--seawater_RFP_LB_NaCl.jpg|400x300px|center]] |
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| + | == Expression and Degradation of Red Fluorescence Protein variants in ΔssrA cells == | ||
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| + | Additional Text | ||
Revision as of 20:09, 29 October 2017
Expression of Red Fluorescence Protein in High Salt Media
(Improved Characterization of parts [http://parts.igem.org/Part:BBa_J04450 J04450], [http://parts.igem.org/Part:BBa_K1399010 K1399010], [http://parts.igem.org/Part:BBa_K1399011 K1399011], and [http://parts.igem.org/Part:BBa_K1399012 K1399012])
As part of our experiments to determine if genetically modified E. coli can grow and thrive in salt water, we plated dilutions of E. coli exposed to different media on agar to obtain CFU/mL counts. We plated them not only on LB + chloramphenicol, but also LB with 3% NaCl (mimicking seawater conditions) to recover any other microorganisms that might be present in the local water mixtures we used. Surprisingly, the E. coli that grew on these plates seemed to not only grow slower, but express RFP to a much lower extent (a decreased fluorescence that was not recovered when given a longer incubation time).
Additional Text
Expression and Degradation of Red Fluorescence Protein variants in ΔssrA cells
Additional Text
