Difference between revisions of "Team:Kingsborough NY/Bootcamp"
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| − | + | The iGEM Bootcamp was a dual outreach and recruitment / training workshop we held over the course of two weeks early in the summer. Students followed a prescibed set of experiments as they got a taste for molecular biology and synthetic biology: attempting to assemble two BioBricks together. | |
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| + | We choose BioBricks representing both RFP and LacZ expression, hoping that the visuals (either Red, Blue, or Dark Purple) would allow students to visualize the efficiency of their cloning. The protocols and experiments are described in detail in our Bootcamp guide, available as a PDF: [[File:T--Kingsborough_NY--iGEM_bootcamp_instructions1.pdf]] | ||
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| + | The results were partly successful: students were able to transform bacteria with ligation reactions and recovery colonies. Moreover, more colonies were recovered for the experimental ligations than the control reactions. However, for some reason the LacZ BioBrick failed to produce the characteristic blue color when grown in the presence of X-gal (we even tested the X-gal with pUC19, and this positive control was blue - data not shown). | ||
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| + | Below are some of the results from one group of Bootcamp students (similar results obtained from other student groups). | ||
Revision as of 03:13, 1 November 2017
The iGEM Bootcamp was a dual outreach and recruitment / training workshop we held over the course of two weeks early in the summer. Students followed a prescibed set of experiments as they got a taste for molecular biology and synthetic biology: attempting to assemble two BioBricks together.
We choose BioBricks representing both RFP and LacZ expression, hoping that the visuals (either Red, Blue, or Dark Purple) would allow students to visualize the efficiency of their cloning. The protocols and experiments are described in detail in our Bootcamp guide, available as a PDF: File:T--Kingsborough NY--iGEM bootcamp instructions1.pdf
The results were partly successful: students were able to transform bacteria with ligation reactions and recovery colonies. Moreover, more colonies were recovered for the experimental ligations than the control reactions. However, for some reason the LacZ BioBrick failed to produce the characteristic blue color when grown in the presence of X-gal (we even tested the X-gal with pUC19, and this positive control was blue - data not shown).
Below are some of the results from one group of Bootcamp students (similar results obtained from other student groups).
