Team:Kingsborough NY/LacZ

Kingsborough Community College || City Unversity of New York || 2017 iGEM Team

Computer Hope

Figure1

Kbcc 3.211111.gif

(Figure1)Shows the plating of K2268005 grown in the dark where the blue indicator shows the direction of plating. The center had the highest expression of LacZ suggesting that rate of cell growth is the highest at the center and rate of cell growth towards the edge of the plate is lower. The cell was induced with 1mM of IPTG in 20 ɥg/ɥl of X-gal.


Figure2

Kbcc mark 3.2dark.jpg

(Figure2)Shows the ImageJ analysis for the average expression level. Each “cell” is a ratio of blue to gray and expression level was found by taking the ratio of the background to the cell.


Figure3

Kbcc light3.2.gif

(Figure3)Shows the plating of K2268005 grown in the light where the blue indicator shows the direction of plating. The center had the highest expression of LacZ suggesting that rate of cell growth is the highest at the center and rate of cell growth towards the edge of the plate is lower. The cell was induced with 1mM of IPTG in 20 ɥg/ɥl of X-gal.

Figure4

Kbcc mark 3.2light.jpg

(Figure4)Shows the ImageJ analysis for the average expression level. Each “cell” is a ratio of blue to gray and expression level was found by taking the ratio of the background to the cell.

Figure5




T--Kingsborough NY--lacZa graph1.png

(Figure5)Shows that there is a significant difference in expression level between K2268005 grown in light and K2268005 grown in dark with significantly higher expression in K2268005 grown in light, p<.001. Data analysis was done by comparing the mean expression level of figure 2 and figure 4 with GraphPad Software.