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...>Testing the performance of phiC31 recombinase using the register assembly construct with the attachment sites PxB.</p>
...tutive expression of phiC31 in the plant. c) Graphic representation of the construct comprised by a promoter and a terminator in opposite directions flanked by
50 KB (6,682 words) - 03:33, 2 November 2017
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<br> (2) Endolysin construct finish
<br> (5) Backbone construct-3NSII, 5NSII,AMP, B0015, and ORI (PCR: dream taq)<br>
26 KB (3,130 words) - 16:14, 28 November 2017
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...rror, so instead we performed 2-step NEB Phusion HF PCR of pSB1C3 with RFP construct</p>
<li>Performed Phusion PCR of pSB1C3 with RFP construct using psB1C3 #2 and the pKDL071 FWD/RVS primers
76 KB (12,349 words) - 20:03, 14 December 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Cross <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
199 KB (29,417 words) - 03:20, 2 November 2017
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...ldenBraid multipartite assembly reaction was performed to create a genetic construct composed by the transcriptional factor, PIF6, fused to a DNA-binding domain
...ression along time for different treatments, including the light-inducible construct (Etr8) under white-light conditions, and a weak constitutive promoter and a
51 KB (6,067 words) - 19:12, 14 December 2017
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.... The construct used for cutting <i>STE2</i> is shown in Figure 2, and the construct used for cutting <i>STE3</i> in Figure 3.
<figcaption><b>Figure 2.</b> gRNA construct for deletion of <i>STE2</i>.
253 KB (35,104 words) - 15:59, 30 October 2017
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<li style="font-family: Rubik">Designed, and began to construct, a variety of framework compatible systems, including a synthetic promoter
...moter was added to enable expression to be under the control of IPTG. This construct was then submitted to IDT for synthesis as a gBlock.
184 KB (26,744 words) - 19:53, 1 November 2017
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...moter was added to enable expression to be under the control of IPTG. This
construct was then submitted to IDT for synthesis as a gBlock.
...replacing the IPTG sensing system in the original detector module with the
construct detailed above, creating part <a href="http://parts.igem.org/Part:BBa_K2205
185 KB (26,866 words) - 22:39, 1 November 2017
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...subject, and also they get information about yeast extraction and protein construct.",,,,Consulted experts to gain more information on cyanobacteria and mathem
...ext two exchanges mainly focused on cooperation</li><li> Help BNU-China to construct a part of the gene circuit, and meanwhile, BNU-China helps with the charact
1.09 MB (163,642 words) - 17:36, 30 October 2017
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<p>Assess robustness of a GFP reporter construct in a range of environments.</p>
...ly. The Golden Gate assembly method successfully produced the GFP reporter construct, however it involved more steps and was more time consuming than Gibson ass
20 KB (2,994 words) - 21:41, 1 November 2017
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...ed split-luciferase system characterized by the 2015 Peking iGEM team, our construct consists of a promoter reporter system that looks at two downstream product
...ia in their intestines are influencing on protein nutrition. We thought to construct a pathway to synthesize amino acids from nitrogen in E.coli, introduce it i
400 KB (63,299 words) - 15:37, 15 August 2018
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...(Kittleson, 2012), which was essential both to obtain P1 particles and to construct each complete CRISPR system.</p>
engineered phagemid would need to become a P1 particle and construct whichever CRISPR system we have inserted into it within an <em>E. coli</em>
25 KB (3,973 words) - 20:43, 25 November 2017
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...lls (control) (15min) or not mixed in the presence or absence of the EL222 construct. Cell mixtures would have then be passed through a particle sizer with a ra
...fluorescence microscopy. We also included a His-Tag within all SpyCatcher construct variants to enable in-vitro analysis of complexes formed with SpyTag varian
122 KB (15,402 words) - 02:31, 2 November 2017
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<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
<td class="col0"><strong>Construct</strong></td><td class="col1">6.43 µg</td>
167 KB (25,036 words) - 21:56, 31 October 2017
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Construct standard structure of integrase int5 and int8.<br>
Construct the expression system of integrase TP901, int2, int3, int4, int5, int7, int
16 KB (1,672 words) - 13:45, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
105 KB (15,409 words) - 02:56, 2 November 2017
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...o take our chances by building a D.I.V.E.R.T. cassette as well as a helper construct (carrying the RT, FLP and the primer cassettes) according to the best of ou
...synthetic regulatory elements<sup>1–3</sup> to keep the synthesized construct short. The synthetic galactose-inducible minimal promoter we used (sequence
68 KB (10,146 words) - 17:37, 15 December 2017
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...ctroporated DNA (BRED) (Figure 1). In this technique a double stranded DNA construct containing the
...to be replaced. The host cell for recombineering of the phage and the DNA construct contains a plasmid conferring genes promoting recombination. The double-str
10 KB (1,666 words) - 23:47, 1 November 2017
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...ycin. The enzyme of this step is BsmBI again. This level is useful, if the construct you are designing requires multiple genes to be transformed into one yeast
...Baker <i>et al.</i>, published in 2017, in which they present a synthetic construct of the Pex5 protein, partly <i>Arabidopsis thaliana</i> and partly <i>Physc
98 KB (14,470 words) - 00:02, 2 November 2017
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<a href="#ConDPR" class="pageNavSm">
Construct Design</a>
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Construct Design</a>
55 KB (6,458 words) - 14:08, 1 November 2017
