Notebook
On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.
Master Mix:
14.3
H2O
114.4
2.5
Buffer
20
1
MgCl2
8
1
dNTP
8
tube:
Label:
0
Control
1
AceF
2
EutG BB
3
YiaY BB
4
AdhP BB
5
Lpd
6
AceE
Master Mix
gDNA
3
24
Buffer
2.5
20
dNTP
1
8
dH2O
13.3
106.4
Taq
0.2
1.6
dH2O
For
Rev
1
4
0.5
0.5
AceF
2
4
0.5
0.5
EutG
3
4
0.5
0.5
YiaY
4
4
0.5
0.5
AdnP
5
4
0.5
0.5
Lpd
6
4
0.5
0.5
AceE
7
5
-
-
-
Master Mix
gDNA
3
24
Buffer
2.5
20
dNTP
1
8
dH2O
12.3
98.4
Taq
0.2
1.6
MgCl2
1
8
dH2O
For
Rev
0
5
-
-
1
4
0.5
0.5
ACE F
2
4
0.5
0.5
EutG
3
4
0.5
0.5
YiaY
4
4
0.5
0.5
AdhP
5
4
0.5
0.5
Lpd
6
4
0.5
0.5
AceE
1
-
DNA(0)
dH2O(5)
2
J23102
3
2
3
GlmY
3
2
4
A(1)
3
2
5/td>
A(2)
3
2
6
B(2)
3
2
7
C(2)
3
2
Max:
Buffer
2.5
20
dNTP
1
8
VR
0.5
4
VF2
0.5
4
Taq
0.2
1.6
dH2O
15.3
122.4
Master Mix
Buffer
2.5
20
dNTP
1
8
VR
0.5
4
VF2
0.5
4
taq
0.2
1.6
dH2O
15.3
122.4
DNA
H2O
1
Control
0u
5u
2
J231013
3
2
3
GlmY-3A
3
2
4
2C
3
2
5
1C
3
2
6
1B
3
2
7
1A
3
2
Master Mix
Buffer
2.5
20
dNTP
1
8
VR
0.5
4
VF2
0.5
4
Taq
0.2
1.6
dH2O
15.3
122.4
DNA
H2O
1
Control
0u
5u
2
J231013
3
2
3
GlmY-3A
3
2
4
2C
3
2
5
1C
3
2
6
1B
3
2
7
1A
3
2
Master Mix
Buffer
2.5
20
dNTP
1
8
VR
0.5
4
VF2
0.5
4
Taq
0.2
1.6
dH2O
15.3
122.4
DNA
H2O
1
Control
0u
5u
2
J23102B pp
3
2
3
J23102 P NNP
3
2
4
GlmY-3A
3
2
5
102B+GlmY 1B
3
2
6
102B+GlmY 1C
3
2
7
102B_GlmY 1D
3
2
Master Mix
H2O
13.3
106.4
Buffer
2.5
20
dNTP
1
8
GDNA
3
24
Taq
0.2
1.6
Gel:
dH2O
For
Rev
1
5
-
-
2
4
0.5
0.5
AceF
3
4
0.5
0.5
EutG
4
4
0.5
0.5
YiaY
5
4
0.5
0.5
AdhP
6
4
0.5
0.5
Lpd
7
4
0.5
0.5
AceE
