Team:Gaston Day School/Notebook

Notebook

On this page, Gaston Day School iGEM team would like to share with you our notes during the experiments from 09/01/2017 to 10/23/2017. The team leader Heena Saqib records the data patiently.

Master Mix:

14.3 H2O 114.4
2.5 Buffer 20
1 MgCl2 8
1 dNTP 8

tube:

2ul H2O
-5ul F
-5ul R

Label:

0 Control
1 AceF
2 EutG BB
3 YiaY BB
4 AdhP BB
5 Lpd
6 AceE

Master Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 13.3 106.4
Taq 0.2 1.6
dH2O For Rev
1 4 0.5 0.5 AceF
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdnP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
7 5 - - -

Master Mix

gDNA 3 24
Buffer 2.5 20
dNTP 1 8
dH2O 12.3 98.4
Taq 0.2 1.6
MgCl2 1 8
dH2O For Rev
0 5 - -
1 4 0.5 0.5 ACE F
2 4 0.5 0.5 EutG
3 4 0.5 0.5 YiaY
4 4 0.5 0.5 AdhP
5 4 0.5 0.5 Lpd
6 4 0.5 0.5 AceE
1 - DNA(0) dH2O(5)
2 J23102 3 2
3 GlmY 3 2
4 A(1) 3 2
5/td> A(2) 3 2
6 B(2) 3 2
7 C(2) 3 2

Max:

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
Taq 0.2 1.6
dH2O 15.3 122.4

Master Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J231013 3 2
3 GlmY-3A 3 2
4 2C 3 2
5 1C 3 2
6 1B 3 2
7 1A 3 2

Master Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
Taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J231013 3 2
3 GlmY-3A 3 2
4 2C 3 2
5 1C 3 2
6 1B 3 2
7 1A 3 2

Master Mix

Buffer 2.5 20
dNTP 1 8
VR 0.5 4
VF2 0.5 4
Taq 0.2 1.6
dH2O 15.3 122.4
DNA H2O
1 Control 0u 5u
2 J23102B pp 3 2
3 J23102 P NNP 3 2
4 GlmY-3A 3 2
5 102B+GlmY 1B 3 2
6 102B+GlmY 1C 3 2
7 102B_GlmY 1D 3 2

Master Mix

H2O 13.3 106.4
Buffer 2.5 20
dNTP 1 8
GDNA 3 24
Taq 0.2 1.6

Gel:

dH2O For Rev
1 5 - -
2 4 0.5 0.5 AceF
3 4 0.5 0.5 EutG
4 4 0.5 0.5 YiaY
5 4 0.5 0.5 AdhP
6 4 0.5 0.5 Lpd
7 4 0.5 0.5 AceE

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