Team:IISER-Pune-India/Basic Part

Basic Parts

Our contribution to the Registry!
IISER Pune India iGEM 2017 Wiki.

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Results

• G13 Promoter; BBa_K228002 G13 is a constitutively expressed mycobacterial promoter obtained from Mycobacterium marinum.
  Ref: The complex architecture of mycobacterial promoters Mae Newton-Foot, Nicolaas C. Gey van Pittius
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• pL-pLeft promoter; BBa_K228000 Hybrid promoter designed by Yash Jawale, which acts as a shuttle promoter between E.coli and Mycobacterium. pL initiates transcription in E.coli, whereas pLeft initiates transcription in Mycobacterium.
  Ref: 1.PL Expression System : A Prokaryotic Expression System, Invitrogen manual 2.Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU
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• pLeft Promoter; BBa_K228001 Constitutive promoter for mycobacterium isolated from mycobacteriophage L5.
  Ref: Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU

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Address

Indian Institute of Science Education and Research,
Dr. Homi Bhabha Road, Pashan,
Maharashtra, India 411008

Social

facebook.com/igemiiserpune
twitter.com/Iiserpune_igem

Email

cathale[at]iiserpune.ac.in
aarti.kejriwal[at]students.iiserpune.ac.in

Phone

(+91) 020 2590-8000

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• Support bacteria, they're the only culture some people have. Design: HTML5 UP & Saumil Shah