Measurement techniques are essential for quantifying and communicating results that are meaningful in comparison to a standard. The iGEM Measurement Committee aims to develop robust measurement procedures for enhancing the quantification and reproducibility of results through the InterLab Study. These results can then be used to reliably characterize biological constructs. For the InterLab Study this year, participating iGEM teams follow a standardized protocol for measuring green fluorescent protein (GFP) using E. coli DH5 cells and a plate reader. Fluorescence data is typically difficult to compare between laboratories because fluorescent measurements are not reported in the same units or the methods of data processing are different. The goal of the InterLab Study is to determine absolute units for measuring GFP that are comparable despite the type of plate reader used to make the measurements. More details about the standardized protocol we used for the study can be found here. Below you will find our data we collected for the InterLab Study using BioTek Synergy 4 plate reader. The InterLab Study can be broken down into two parts: plate reader calibration and cell measurement. Below you will find the data we collected for the InterLab Study using the BioTek Synergy 4 plate reader.