Difference between revisions of "Team:Bielefeld-CeBiTec/Project/translational system/library and selection"
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<p class="figure subtitle"><b>Figure 2: Adaption of the aminoacyl-synthetase.</b><br> Positive and negative selection for the specificity of the orthogonal synthetase for the ncAA in <i> E. coli</i>. </p> | <p class="figure subtitle"><b>Figure 2: Adaption of the aminoacyl-synthetase.</b><br> Positive and negative selection for the specificity of the orthogonal synthetase for the ncAA in <i> E. coli</i>. </p> | ||
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Revision as of 02:38, 27 August 2017
Library and Selection
Tyrosine and Pyrosyl-Lysine tRNA/aminoacyl-synthetase
Tyrosine tRNA/aminoacyl-synthetase
Figure 1: Tyrosine-binding site in apo M. jannaschii TyrRS
The electrostatic distribution around the tyrosine-binding site is shown. Positive potential is blue (10 mV), neutral potential (0 mV) is white, and negative potential (−10 mV) is red (Zhang et al., 2005).
Pyrosyl-Lysine tRNA/aminoacyl-synthetase
Generating the Library
Selection
Modification of the aaRS
Figure 2: Adaption of the aminoacyl-synthetase.
Positive and negative selection for the specificity of the orthogonal synthetase for the ncAA in E. coli.