Difference between revisions of "Team:UNOTT/Design2"
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<p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/4/4c/UNOTT2017-bricks.png" alt="" width="447" height="182" /> </p> | <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/4/4c/UNOTT2017-bricks.png" alt="" width="447" height="182" /> </p> | ||
<p style="text-align: center;"><span style="color: #ffffff;"> </span></p> | <p style="text-align: center;"><span style="color: #ffffff;"> </span></p> | ||
| − | <p>These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator. This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal. | + | <p>These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator "T". This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal. |
<h3 style="text-align: center;"><span style="color: #D74214;"> Brick stitching </span></h3> | <h3 style="text-align: center;"><span style="color: #D74214;"> Brick stitching </span></h3> | ||
<p style="text-align: center;"><span style="color: #ffffff;">___________________________</span></p> | <p style="text-align: center;"><span style="color: #ffffff;">___________________________</span></p> | ||
<p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/2/29/UNOTT2017-DCBAbricks.png" alt="" width="500" height="128" /> </p> | <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/2/29/UNOTT2017-DCBAbricks.png" alt="" width="500" height="128" /> </p> | ||
<p>These bricks are then stitched together via amplifying each randomly assembled brick through common amplification sites and then cutting them using a set of restriction enzymes which give each plasmid a specific order of bricks, depending on which are cut and then ligated together. As shown. | <p>These bricks are then stitched together via amplifying each randomly assembled brick through common amplification sites and then cutting them using a set of restriction enzymes which give each plasmid a specific order of bricks, depending on which are cut and then ligated together. As shown. | ||
| + | <h3 style="text-align: center;"><span style="color: #D74214;"> Plasmid Design </span></h3> | ||
| + | <p style="text-align: center;"><span style="color: #ffffff;">___________________________</span></p> | ||
| + | <p style="text-align: center;"><img src="https://static.igem.org/mediawiki/2017/6/67/UNOTT2017-jkeydesign.png" alt="" width="800" height="400" /> </p> | ||
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Revision as of 00:13, 14 September 2017
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KEY PLASMID DESIGN
Random Brick formation (Components of plasmid)
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These bricks are formed from a random soup of characterised promoters "P", a reporter gene fluorescent protein, and a random terminator "T". This uses BSA I sites already digested previously into the DNA in order to ligate randomly in the correct order to create a random, yet purposeful and functional fluorescent signal.
Brick stitching
___________________________
These bricks are then stitched together via amplifying each randomly assembled brick through common amplification sites and then cutting them using a set of restriction enzymes which give each plasmid a specific order of bricks, depending on which are cut and then ligated together. As shown.
Plasmid Design
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