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The AzoF-RS was provided by CU Boulder and deposited in the Registry of Standard Biological Parts as BBa_X. They got it from the Schultz lab, which performed a selection experiment on the M. jannaschii TyrRS to evolve a new aaRS capable of incorporating the photoisomerizable phenylalanine-4‘-azobenzene (AzoF). Figure 1 shows a sequence alignment of the protein sequences of the M. jannaschii TyrRS and the AzoF-RS after the selection process. | The AzoF-RS was provided by CU Boulder and deposited in the Registry of Standard Biological Parts as BBa_X. They got it from the Schultz lab, which performed a selection experiment on the M. jannaschii TyrRS to evolve a new aaRS capable of incorporating the photoisomerizable phenylalanine-4‘-azobenzene (AzoF). Figure 1 shows a sequence alignment of the protein sequences of the M. jannaschii TyrRS and the AzoF-RS after the selection process. | ||
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+ | <img class="figure image" src="https://static.igem.org/mediawiki/2017/b/b0/T--Bielefeld-CeBiTec--YKE_AzoF_Alignment.png"> | ||
+ | <p class="figure subtitle"><b>Figure 1: Sequence alignment of the M. jannaschii TyrRS and the AzoF-RS of the Schultz lab. The alignment shows six differences in the protein sequences. </b><p> | ||
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Revision as of 10:48, 4 October 2017
Photoswitching
Design of AzoF-RS
Figure 1: Sequence alignment of the M. jannaschii TyrRS and the AzoF-RS of the Schultz lab. The alignment shows six differences in the protein sequences.