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<img class="figure image" src="https://static.igem.org/mediawiki/2017/4/4c/T--Bielefeld-CeBiTec--YKE_lycopene_preexperiment.jpg"> | <img class="figure image" src="https://static.igem.org/mediawiki/2017/4/4c/T--Bielefeld-CeBiTec--YKE_lycopene_preexperiment.jpg"> | ||
<p class="figure subtitle"><b>Figure 2: Cell pellets of the functional CrtI-variant (left), the amber318 (middle) and the amber353 (right) variants vortexed in 500 µl acetone. </b><p> | <p class="figure subtitle"><b>Figure 2: Cell pellets of the functional CrtI-variant (left), the amber318 (middle) and the amber353 (right) variants vortexed in 500 µl acetone. </b><p> | ||
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+ | We then extracted the lycopene from the pellet to quantify the amount of lycopene produced by the three cultures. For that, we resuspended the pellet in 400 µl acetone and vortexed it to solve the lycopene. We then added 400 µl water and made and absorbance measurement. We first made an absorbance spectrum to identify the best wavelength for the quantification (Figure 3). | ||
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+ | <img class="figure image" src="https://static.igem.org/mediawiki/2017/f/fe/T--Bielefeld-CeBiTec--YKE_lycopene_spectrum.png"> | ||
+ | <p class="figure subtitle"><b>Figure 3: Absorbance spectrum of the positive lycopene sample from 400 to 550 nm normalized with the measurement of a 1:1 acetone water sample.</b><p> | ||
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<div class="bevel bl"></div> | <div class="bevel bl"></div> | ||
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Revision as of 10:56, 4 October 2017
Photoswitching
Design of AzoF-RS
Figure 1: Sequence alignment of the M. jannaschii TyrRS and the AzoF-RS of the Schultz lab. The alignment shows six differences in the protein sequences.
Two Amber-CrtI-Variants
Figure 2: Cell pellets of the functional CrtI-variant (left), the amber318 (middle) and the amber353 (right) variants vortexed in 500 µl acetone.
Figure 3: Absorbance spectrum of the positive lycopene sample from 400 to 550 nm normalized with the measurement of a 1:1 acetone water sample.