Difference between revisions of "Team:UCSC/Safety"
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<h2>PROJECT SAFETY</h2> | <h2>PROJECT SAFETY</h2> | ||
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| + | <p>Vitamin B-12 Safety | ||
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| + | ssuE => produces FMN reductase | ||
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| + | The synthesis of 5,6-DMB first requires the presence of reduced flavin mononucleotide (FMNH2). FMNH2 is reduced from flavin mononucleotide (FMN) by FMN reductase, which is encoded with the ssuE gene [19, 20]. | ||
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| + | organism: synechoccocous 7002 | ||
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| + | FMNH2 + NADP+ = FMN + NADPH | ||
| + | |||
| + | bluB =></p> | ||
</div> | </div> | ||
Revision as of 22:47, 4 October 2017
SAFETY
When choosing an organism to help us produce acetaminophen and vitamin B-12 we sought out an FDA approved microbe; however, we resorted to using a non-toxic cyanobacteria as a model for spirulina. Spirulina is a generally recognized as safe organism and our genes were chosen from bio-safety level 1 organisms.
LAB SAFETY
Proper PPE for bio-safety level 1 organisms are worn in our labratory. Within the lab space, every person is expected to be wearing gloves, a labcoat, and protective goggles. All personnel also must be accompanied with one other person so there is no one alone in lab.
PROJECT SAFETY
Vitamin B-12 Safety ssuE => produces FMN reductase The synthesis of 5,6-DMB first requires the presence of reduced flavin mononucleotide (FMNH2). FMNH2 is reduced from flavin mononucleotide (FMN) by FMN reductase, which is encoded with the ssuE gene [19, 20]. organism: synechoccocous 7002 FMNH2 + NADP+ = FMN + NADPH bluB =>
