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<div class="contentbox"> | <div class="contentbox"> | ||
− | + | <div class="content"> | |
− | + | <h3> Protocols </h3> | |
− | + | <ul> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2017/8/85/T--Bielefeld-CeBiTec--protocol_50xTAE.pdf" target="blank">50xTAE Stock Solution</a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2017/1/15/T--Bielefeld-CeBiTec--protocol_acetone.pdf" target="blank">Acetone Precipitation</a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2017/f/fe/T--Bielefeld-CeBiTec--protocol_biotynilation.pdf" target="blank">Biotynilation of glass slides</a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2017/3/3a/T--Bielefeld-CeBiTec--protocol_blunt-end-ligation.pdf" target="blank">Blunt end ligation</a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2017/c/cc/T--Bielefeld-CeBiTec--protocol_colonyPCR_GoTaq_G2.pdf" target="blank">Colony PCR GoTaq® G2 DNA Polymerase</a></li> | |
− | + | <li><a href="https://static.igem.org/mediawiki/2017/a/ab/T--Bielefeld-CeBiTec--protocol_electrotrafo.pdf" target="blank">Transformation via electroporation</a></li> | |
− | + | <li><a href="" target="blank">Fast cell lysis buffer</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | <td><a href=" | + | <li><a href="" target="blank">Lorem</a></li> |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | <li><a href="" target="blank">Lorem</a></li> | |
− | + | </ul> | |
− | + | <h3> Enzymes </h3> | |
− | + | <h4> Digestion enzymes </h4> | |
− | + | <table> | |
− | </div> | + | <thead> |
+ | <tr> | ||
+ | <th>Enzyme</th> | ||
+ | <th>Producer</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r3552-agei-hf"><i>Age</i>I-HF</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0535-bsai"><i>Bsa</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0176-dpni"><i>Dpn</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.jenabioscience.com/molecular-biology/restriction-enzymes/d-enzymes/en-160-dpni"><i>Dpn</i>I</a></td> | ||
+ | <td>Jena Bioscience</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0590-ecii"><i>Eci</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.thermofisher.com/order/catalog/product/ER0301"><i>Eco</i>RV</a></td> | ||
+ | <td>Fermentas</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0163-mnli"><i>Mnl</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.jenabioscience.com/molecular-biology/restriction-enzymes/n-enzymes/en-124-noti"><i>Not</i>I</a></td> | ||
+ | <td>Jena Bioscience</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0140-psti"><i>Pst</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.thermofisher.com/order/catalog/product/ER0611"><i>Pst</i>I</a></td> | ||
+ | <td>Thermo Fisher Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r3140-psti-hf"><i>Pst</i>I-HF</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0569-sapi"><i>Sap</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0133-spei"><i>Spe</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/r0145-xbai"><i>Xba</i>I</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.jenabioscience.com/molecular-biology/restriction-enzymes/x-enzymes/en-143-xbai"><i>Xba</i>I</a></td> | ||
+ | <td>Jena Bioscience</td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | <h4> Polymerases </h4> | ||
+ | <table> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Enzyme</th> | ||
+ | <th>Producer</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.promega.de/products/pcr/endpoint-pcr/gotaq-dna-polymerase/?catNum=M3001">Go Taq DNA polymerase</a></td> | ||
+ | <td>Promega</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.thermofisher.com/order/catalog/product/EP0054">Klenow Fragment</a></td> | ||
+ | <td>Thermo Fisher Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.promega.de/products/pcr/endpoint-pcr/gotaq-dna-polymerase/?catNum=M3001">Phusion High-Fidelity DNA polymerase </a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/m0491-q5-high-fidelity-dna-polymerase">Q5 High-Fidelity DNA polymerase </a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.jenabioscience.com/molecular-biology/standard-pcr/ready-to-use-mixes/pcr-108-red-load-taq-master">Taq Polymerase </a></td> | ||
+ | <td>Jena Bioscience</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | <h4> Other Enzymes </h4> | ||
+ | <table> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <th>Enzyme</th> | ||
+ | <th>Producer</th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.jenabioscience.com/molecular-biology/modifying-enzymes/ligases-phosphatases-kinases/en-174-shrimp-alkaline-phosphatase-rsap">Shrimp Alkaline Phosphatase</a></td> | ||
+ | <td>Jena Bioscience</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/m0202-t4-dna-ligase">T4 DNA Ligase</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.thermofisher.com/order/catalog/product/15224017">T4 DNA Ligase</a></td> | ||
+ | <td>Thermo Fisher Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="https://www.neb.com/products/m0201-t4-polynucleotide-kinase">T4 Polynucleotide Kinase</a></td> | ||
+ | <td>NEB</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
Revision as of 19:46, 10 October 2017
Methods
Protocols
- 50xTAE Stock Solution
- Acetone Precipitation
- Biotynilation of glass slides
- Blunt end ligation
- Colony PCR GoTaq® G2 DNA Polymerase
- Transformation via electroporation
- Fast cell lysis buffer
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
- Lorem
Enzymes
Digestion enzymes
Enzyme | Producer |
---|---|
AgeI-HF | NEB |
BsaI | NEB |
DpnI | NEB |
DpnI | Jena Bioscience |
EciI | NEB |
EcoRV | Fermentas |
MnlI | NEB |
NotI | Jena Bioscience |
PstI | NEB |
PstI | Thermo Fisher Scientific |
PstI-HF | NEB |
SapI | NEB |
SpeI | NEB |
XbaI | NEB |
XbaI | Jena Bioscience |
Polymerases
Enzyme | Producer |
---|---|
Go Taq DNA polymerase | Promega |
Klenow Fragment | Thermo Fisher Scientific |
Phusion High-Fidelity DNA polymerase | NEB |
Q5 High-Fidelity DNA polymerase | NEB |
Taq Polymerase | Jena Bioscience |
Other Enzymes
Enzyme | Producer |
---|---|
Shrimp Alkaline Phosphatase | Jena Bioscience |
T4 DNA Ligase | NEB |
T4 DNA Ligase | Thermo Fisher Scientific |
T4 Polynucleotide Kinase | NEB |