Line 76: | Line 76: | ||
This peptide linker is fused to | This peptide linker is fused to | ||
our actual detectable unit, the methylcoumarin.</p> | our actual detectable unit, the methylcoumarin.</p> | ||
− | <img src="https://static.igem.org/mediawiki/2017/4/4a/T--TU_Darmstadt--Flourophor_linken.jpg" /> | + | <center><img src="https://static.igem.org/mediawiki/2017/4/4a/T--TU_Darmstadt--Flourophor_linken.jpg" /></center> |
<p>This linker was chosen due to the fact that chymotrysin cleaves peptides n-terminal of aromatic amino acids.</p> | <p>This linker was chosen due to the fact that chymotrysin cleaves peptides n-terminal of aromatic amino acids.</p> | ||
− | <img src="https://static.igem.org/mediawiki/2017/2/25/T--TU_Darmstadt--cleavedge.jpg" /> | + | <center><img src="https://static.igem.org/mediawiki/2017/2/25/T--TU_Darmstadt--cleavedge.jpg" /></center> |
<p>In our studies, we present an uncomplicated method to repeat the work of Ebrahimi and Schönherr in a way that works for iGEMers and FabLabers. | <p>In our studies, we present an uncomplicated method to repeat the work of Ebrahimi and Schönherr in a way that works for iGEMers and FabLabers. | ||
For that, we combined a few instructions to guarantee a working product without the need of expensive instrumental analysis.</p> | For that, we combined a few instructions to guarantee a working product without the need of expensive instrumental analysis.</p> |
Revision as of 18:25, 13 October 2017