Difference between revisions of "Team:WashU StLouis/Notebook"

Line 525: Line 525:
 
         <li> Prepared LB Media </li>
 
         <li> Prepared LB Media </li>
 
         <li> Re-suspended DNA (<em>D. Radiodurans</em> Uracil DNA Glycosylate 2) from iGEM kit </li>
 
         <li> Re-suspended DNA (<em>D. Radiodurans</em> Uracil DNA Glycosylate 2) from iGEM kit </li>
         <li> Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells </li>
+
         <li> Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) </li>
 
         <li> Prepared LB Agar </li>
 
         <li> Prepared LB Agar </li>
 
         <li> Prepared overnight culture to make competent cells (MHD42) </li>
 
         <li> Prepared overnight culture to make competent cells (MHD42) </li>

Revision as of 20:16, 6 June 2017

Notebook

June 2017
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25 26 27 28 29 30

Micah, Maddie, Alex

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Micah, Maddie

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

  1. Prepared Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar

Micah, Maddie, Alex, Mark, Collin

  1. Prepared DH5α competent cells
  2. Tested MHD42 cells for competency
  3. Tested DH5α cells for competency