Team:WashU StLouis/Parts

Our team designed several parts that are intended to confer resistance to UV-B radiation in E. coli. Our basic parts encode radiation-resistance proteins, while our composite parts combine these proteins with an inducible Lac reporter and blue chromoprotein, a reporter.

Basic Parts

These basic parts are all coding sequences that came from foreign organisms, and we hoped would confer radiation resistance to E. Coli and our Cyanobacteria strain

Part Name	Part Number	Part Function
Dsup	BBa_K2195000	Tardigrade DNA binding protein, provides radiation resistance
phrAc	BBa_K2195001	Cyanobacteria DNA photolyase, provides radiation resistance
phrAt	BBa_K2195006	Tardigrade DNA photolyase, provides radiation resistance

Composite Parts

Two of our composite parts contain Lac-inducible forms of our resistance genes. One is a reporter using the Blue Chromoprotein that we used as a control. The last is an antibiotic resistance gene that we improved by optimizing for E. Coli

Part Name	Part Number	Part Function
Dsup Full Gene	BBa_K2195005	Synthesized gene of Lac Promoter, RBS and Dsup
uvsE Full Gene	BBa_K2195008	Synthesized gene of Lac Promoter, RBS and uvsE
Blue Chromoprotein Reporter	BBa_K2195007	Synthesized gene of Lac Promoter, RBS and BCP
Neomycin Phosphotransferase full gene	BBa_K2195002	Confers antibiotic resistance to Kanamycin and Neomycin