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Revision as of 03:42, 7 June 2017

Notebook

June 2017
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Micah, Maddie, Alex

Lab Work

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Outside Work / Discussion

Micah, Maddie

Lab Work

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

Outside Work / Discussion

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar

Outside Work / Discussion

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared DH5α competent cells
  2. Tested MHD42 cells for competency
  3. Tested DH5α cells for competency

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion

Lab Work

Outside Work / Discussion