Difference between revisions of "Team:WashU StLouis/Notebook"

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     <p><strong> Lab Work </strong></p>
 
     <p><strong> Lab Work </strong></p>
 
     <ol>
 
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         <li> Prepared Chloramphenicol (CM) plates </li>
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         <li> Prepared LB + Chloramphenicol (CM) plates </li>
 
         <li> Prepared Ampicillin solution to be used for plates in the future </li>
 
         <li> Prepared Ampicillin solution to be used for plates in the future </li>
 
         <li> Made more LB Agar </li>
 
         <li> Made more LB Agar </li>
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     <p><strong> Outside Work / Discussion </strong><p>
 
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Revision as of 17:22, 7 June 2017

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Notebook

June 2017
1 2 3
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11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

Micah, Maddie, Alex

Lab Work

  1. Prepared LB Media
  2. Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
  3. Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
  4. Prepared LB Agar
  5. Prepared overnight culture to make competent cells (MHD42)

Outside Work / Discussion

Micah, Maddie

Lab Work

  1. Prepared MHD42 competent cells
  2. Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
    • Transformation was a success with 100 colonies
    • Plate was stored in 4 deg. Celsius for future use
  3. Prepared TSS Buffer

Outside Work / Discussion

No Work!

No Work!

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared LB + Chloramphenicol (CM) plates
  2. Prepared Ampicillin solution to be used for plates in the future
  3. Made more LB Agar

Outside Work / Discussion

Micah, Maddie, Alex, Mark, Collin

Lab Work

  1. Prepared DH5α competent cells
  2. Tested MHD42 cells for competency
  3. Tested DH5α cells for competency

Outside Work / Discussion

Lab Work

  1. Prepared LB + Ampicillin (Amp) plates

Outside Work / Discussion

Lab Work

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Lab Work

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Lab Work

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Lab Work

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Lab Work

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Lab Work

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Lab Work

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Lab Work

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