Difference between revisions of "Team:WashU StLouis/Notebook"
| Line 612: | Line 612: | ||
</ul> | </ul> | ||
<li> Prepared LB + Ampicillin (Amp) plates </li> | <li> Prepared LB + Ampicillin (Amp) plates </li> | ||
| − | <li> </li> | + | <li> Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits</li> |
| − | <li> </li> | + | <li> Transformed part BBa_B0034 in plasmid psB1A2 in to MHD42 competent cells </li> |
<li> </li> | <li> </li> | ||
</ol> | </ol> | ||
Revision as of 04:19, 8 June 2017
Notebook
| June 2017 | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | |
Micah, Maddie, Alex
Lab Work
- Prepared LB Media
- Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit
- Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation)
- Prepared LB Agar
- Prepared overnight culture to make competent cells (MHD42)
Outside Work / Discussion
Micah, Maddie
Lab Work
- Prepared MHD42 competent cells
- Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2
- Transformation was a success with 100 colonies
- Plate was stored in 4 deg. Celsius for future use
- Prepared TSS Buffer
Outside Work / Discussion
No Work!
No Work!
Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared LB + Chloramphenicol (CM) plates
- Prepared Ampicillin solution to be used for plates in the future
- Made more LB Agar
Outside Work / Discussion
Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared DH5α competent cells
- Tested MHD42 cells for competency
- Tested DH5α cells for competency
Outside Work / Discussion
Lab Work
- Checked competency test for MHD42 cells
- Test was successful!
-
- Checked competency test for DH5(alpha)
- Test showed that the cells were competent but had a low transformation efficiency
-
- Prepared LB + Ampicillin (Amp) plates
- Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits
- Transformed part BBa_B0034 in plasmid psB1A2 in to MHD42 competent cells
Outside Work / Discussion
Lab Work
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Lab Work
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