Difference between revisions of "Team:WashU StLouis/Notebook"
| Line 522: | Line 522: | ||
<div class="mySlides fade"> | <div class="mySlides fade"> | ||
<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
| − | <p><em>Micah, Maddie, Alex</em></p> | + | <p><em>Present: Micah, Maddie, Alex</em></p> |
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared LB Media </li> | + | <li> Prepared LB Media <em>(Micah, Maddie, Alex)</em></li> |
| − | <li> Re-suspended DNA (<em>D. Radiodurans</em> Uracil DNA Glycosylate 2) from iGEM kit </li> | + | <li> Re-suspended DNA (<em>D. Radiodurans</em> Uracil DNA Glycosylate 2) from iGEM kit <em>(Micah, Maddie, Alex)</em></li> |
| − | <li> Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) </li> | + | <li> Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) <em>(Micah Maddie, Alex)</em></li> |
| − | <li> Prepared LB Agar </li> | + | <li> Prepared LB Agar <em>(Micah, Maddie, Alex)</em></li> |
| − | <li> Prepared overnight culture to make competent cells (MHD42) </li> | + | <li> Prepared overnight culture to make competent cells (MHD42) <em>(Micah, Maddie, Alex)</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
| Line 541: | Line 541: | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared MHD42 competent cells </li> | + | <li> Prepared MHD42 competent cells <em>(Micah, Maddie)</em></li> |
| − | <li> Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 </li> | + | <li> Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 <em>(Micah, Maddie)</em></li> |
<ul> | <ul> | ||
<li> Transformation was a success with 100 colonies </li> | <li> Transformation was a success with 100 colonies </li> | ||
<li> Plate was stored in 4 deg. Celsius for future use </li> | <li> Plate was stored in 4 deg. Celsius for future use </li> | ||
</ul> | </ul> | ||
| − | <li> Prepared TSS Buffer </li> | + | <li> Prepared TSS Buffer <em>(Micah, Maddie)</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
| Line 573: | Line 573: | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared LB + Chloramphenicol (CM) plates </li> | + | <li> Prepared LB + Chloramphenicol (CM) plates <em>(Micah, Maddie, Mark, Collin)</em></li> |
| − | <li> Prepared Ampicillin solution to be used for plates in the future </li> | + | <li> Prepared Ampicillin solution to be used for plates in the future <em>(Micah, Maddie, Mark, Collin)</em></li> |
| − | <li> Made more LB Agar </li> | + | <li> Made more LB Agar <em>(Alex)</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
<ol> | <ol> | ||
| − | <li> Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene </li> | + | <li> Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
| − | <li> Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) </li> | + | <li> Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
| − | <li> Met with Dr. Brennan to discuss updates </li> | + | <li> Met with Dr. Brennan to discuss updates <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
</ol> | </ol> | ||
</div> | </div> | ||
| Line 592: | Line 592: | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Prepared DH5α competent cells</li> | + | <li> Prepared DH5α competent cells <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
| − | <li> Tested MHD42 cells for competency</li> | + | <li> Tested MHD42 cells for competency <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
| − | <li> Tested DH5α cells for competency</li> | + | <li> Tested DH5α cells for competency <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
<ol> | <ol> | ||
| − | <li> Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day </li> | + | <li> Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
<ul> | <ul> | ||
<li> The <em>Deinococcus radiodurans</em> UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes. </li> | <li> The <em>Deinococcus radiodurans</em> UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes. </li> | ||
</ul> | </ul> | ||
| − | <li> Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) </li> | + | <li> Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
</ol> | </ol> | ||
</div> | </div> | ||
| Line 613: | Line 613: | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
<ol> | <ol> | ||
| − | <li> Checked competency test for MHD42 cells</li> | + | <li> Checked competency test for MHD42 cells <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
<ul> | <ul> | ||
<li> Test was successful! </li> | <li> Test was successful! </li> | ||
<li> <img src="https://static.igem.org/mediawiki/2017/0/0c/T--WashU_StLouis--2017-06-07_MHD42_Comp_Cells_Test_Results.jpg"/></li> | <li> <img src="https://static.igem.org/mediawiki/2017/0/0c/T--WashU_StLouis--2017-06-07_MHD42_Comp_Cells_Test_Results.jpg"/></li> | ||
</ul> | </ul> | ||
| − | <li> Checked competency test for DH5α </li> | + | <li> Checked competency test for DH5α <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
<ul> | <ul> | ||
<li> Test showed that the cells were competent but had a low transformation efficiency </li> | <li> Test showed that the cells were competent but had a low transformation efficiency </li> | ||
<li> <img src="https://static.igem.org/mediawiki/2017/a/ae/T--WashU_StLouis--2017-06-07_DH5alpha_Comp_Cells_Test_Results.jpg"/> </li> | <li> <img src="https://static.igem.org/mediawiki/2017/a/ae/T--WashU_StLouis--2017-06-07_DH5alpha_Comp_Cells_Test_Results.jpg"/> </li> | ||
</ul> | </ul> | ||
| − | <li> Prepared LB + Ampicillin (Amp) plates </li> | + | <li> Prepared LB + Ampicillin (Amp) plates <em>(Micah, Mark, Collin)</em></li> |
| − | <li> Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits</li> | + | <li> Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits <em>(Maddie, Alex)</em></li> |
| − | <li> Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells </li> | + | <li> Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells <em>(Maddie, Alex)</em></li> |
| − | <li> Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells </li> | + | <li> Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells <em>(Maddie, Alex)</em></li> |
| − | <li> Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells </li> | + | <li> Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells <em>(Maddie, Alex)</em></li> |
| − | <li> Prepared overnight culture of DH5α competent cells </li> | + | <li> Prepared overnight culture of DH5α competent cells <em>(Mark, Collin)</em></li> |
</ol> | </ol> | ||
<p><strong> Outside Work / Discussion </strong><p> | <p><strong> Outside Work / Discussion </strong><p> | ||
<ol> | <ol> | ||
| − | <li> Discussed modelling ideas </li> | + | <li> Discussed modelling ideas <em>(Micah, Maddie, Mark, Collin)</em></li> |
| − | <li> Learned how to design primers from Eugene </li> | + | <li> Learned how to design primers from Eugene <em>(Micah, Maddie, Mark, Collin, Alex)</em></li> |
</ol> | </ol> | ||
</div> | </div> | ||
Revision as of 05:06, 8 June 2017
Notebook
| June 2017 | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | |
Present: Micah, Maddie, Alex
Lab Work
- Prepared LB Media (Micah, Maddie, Alex)
- Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit (Micah, Maddie, Alex)
- Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) (Micah Maddie, Alex)
- Prepared LB Agar (Micah, Maddie, Alex)
- Prepared overnight culture to make competent cells (MHD42) (Micah, Maddie, Alex)
Outside Work / Discussion
Micah, Maddie
Lab Work
- Prepared MHD42 competent cells (Micah, Maddie)
- Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 (Micah, Maddie)
- Transformation was a success with 100 colonies
- Plate was stored in 4 deg. Celsius for future use
- Prepared TSS Buffer (Micah, Maddie)
Outside Work / Discussion
No Work!
No Work!
Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared LB + Chloramphenicol (CM) plates (Micah, Maddie, Mark, Collin)
- Prepared Ampicillin solution to be used for plates in the future (Micah, Maddie, Mark, Collin)
- Made more LB Agar (Alex)
Outside Work / Discussion
- Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene (Micah, Maddie, Mark, Collin, Alex)
- Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) (Micah, Maddie, Mark, Collin, Alex)
- Met with Dr. Brennan to discuss updates (Micah, Maddie, Mark, Collin, Alex)
Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared DH5α competent cells (Micah, Maddie, Mark, Collin, Alex)
- Tested MHD42 cells for competency (Micah, Maddie, Mark, Collin, Alex)
- Tested DH5α cells for competency (Micah, Maddie, Mark, Collin, Alex)
Outside Work / Discussion
- Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day (Micah, Maddie, Mark, Collin, Alex)
- The Deinococcus radiodurans UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes.
- Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) (Micah, Maddie, Mark, Collin, Alex)
Lab Work
- Checked competency test for MHD42 cells (Micah, Maddie, Mark, Collin, Alex)
- Test was successful!
-
- Checked competency test for DH5α (Micah, Maddie, Mark, Collin, Alex)
- Test showed that the cells were competent but had a low transformation efficiency
-
- Prepared LB + Ampicillin (Amp) plates (Micah, Mark, Collin)
- Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits (Maddie, Alex)
- Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells (Maddie, Alex)
- Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells (Maddie, Alex)
- Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells (Maddie, Alex)
- Prepared overnight culture of DH5α competent cells (Mark, Collin)
Outside Work / Discussion
- Discussed modelling ideas (Micah, Maddie, Mark, Collin)
- Learned how to design primers from Eugene (Micah, Maddie, Mark, Collin, Alex)
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
Outside Work / Discussion
Lab Work
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Lab Work
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Lab Work
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Lab Work
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Lab Work
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