Difference between revisions of "Team:WashU StLouis/Notebook"
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| − | img src="https://static.igem.org/mediawiki/2017/1/14/T--WashU_StLouis--2017-06-08_BBa_K1499200_in_MHD42_Transformation.jpg" style="height:200px"> | + | <img src="https://static.igem.org/mediawiki/2017/1/14/T--WashU_StLouis--2017-06-08_BBa_K1499200_in_MHD42_Transformation.jpg" style="height:200px;width:200px"> |
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Revision as of 22:25, 8 June 2017
Notebook
Click on a date to see what we did on that day! Click the double arrowheads to switch months.
| June 2017 | ||||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | |
Present: Micah, Maddie, Alex
Lab Work
- Prepared LB Media [Micah, Maddie, Alex]
- Re-suspended DNA (D. Radiodurans Uracil DNA Glycosylate 2) from iGEM kit [Micah, Maddie, Alex]
- Transformed Uracil DNA Glycosylate 2 in psB1C3 plasmid into MHD42 competent cells (Practice transformation) [Micah, Maddie, Alex]
- Prepared LB Agar [Micah, Maddie, Alex]
- Prepared overnight culture to make competent cells (MHD42) [Micah, Maddie, Alex]
Outside Work / Discussion
Present: Micah, Maddie
Lab Work
- Prepared MHD42 competent cells [Micah, Maddie]
- Checked on transformed MHD42 cells with Uracil DNA Glycosylate 2 [Micah, Maddie]
- Transformation was a success with 100 colonies
- Plate was stored in 4 deg. Celsius for future use
- Prepared TSS Buffer [Micah, Maddie]
Outside Work / Discussion
No Work!
No Work!
Present: Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared LB + Chloramphenicol (CM) plates [Micah, Maddie, Mark, Collin]
- Prepared Ampicillin solution to be used for plates in the future [Micah, Maddie, Mark, Collin]
- Made more LB Agar [Alex]
Outside Work / Discussion
- Discussed shifting focus from Dsup gene to a variety of UV radiation resistance gene [Micah, Maddie, Mark, Collin, Alex]
- Found more genes to compare to Dsup (i.e. phrA in tardigrades and cyanobacteria) [Micah, Maddie, Mark, Collin, Alex]
- Met with Dr. Brennan to discuss updates [Micah, Maddie, Mark, Collin, Alex]
Present: Micah, Maddie, Alex, Mark, Collin
Lab Work
- Prepared DH5α competent cells [Micah, Maddie, Mark, Collin, Alex]
- Tested MHD42 cells for competency [Micah, Maddie, Mark, Collin, Alex]
- Tested DH5α cells for competency [Micah, Maddie, Mark, Collin, Alex]
Outside Work / Discussion
- Fleshed out project and decided specific parts to transform [BBa_B0034 (RBS), BBa_R0010 (lac promoter), and BBa_K1499200 (uvsE gene)] into MHD42 cells during the next day [Micah, Maddie, Mark, Collin, Alex]
- The Deinococcus radiodurans UV DNA damage endonuclease (uvsE) gene is a UV radiation damage repair gene from the Stanford-Brown-Spelman 2014 team. We plan to use this to compare to other UV radiation repair genes.
- Planned out more long-term goals such as collaborations with other teams (especially those in the Midwest) [Micah, Maddie, Mark, Collin, Alex]
Present: Micah, Maddie, Mark, Collin, Alex
Lab Work
- Checked competency test for MHD42 cells [Micah, Maddie, Mark, Collin, Alex]
- Test was successful!
-
- Checked competency test for DH5α [Micah, Maddie, Mark, Collin, Alex]
- Test showed that the cells were competent but had a low transformation efficiency
-
- Prepared LB + Ampicillin (Amp) plates [Micah, Mark, Collin]
- Resuspeded parts BBa_B0034, BBa_R0010, and BBa_K1499200 from current and past distribution kits [Maddie, Alex]
- Transformed part BBa_B0034 (with plasmid psB1A2) into MHD42 competent cells [Maddie, Alex]
- Transformed part BBa_R0010 (with plasmid psB1C3) into MHD42 competent cells [Maddie, Alex]
- Transformed part BBa_K1499200 (with plasmid psB1C3) into MHD42 competent cells [Maddie, Alex]
- Prepared overnight culture of DH5α competent cells [Mark, Collin]
Outside Work / Discussion
- Discussed modelling ideas [Micah, Maddie, Mark, Collin]
- Learned how to design primers from Eugene [Micah, Maddie, Mark, Collin, Alex]
Present: Alex, Maddie, Micah, Collin, Mark, Zoe
Lab Work
- Checked the results of the transformations of BBa_B0034, BBa_R0010, and BBa_K1499200 into MHD42 cells [Alex, Micah, Zoe]
-
- Made 900 mL of LB media [Collin, Mark, Zoe]
- Prepared DH5α competent cells [Micah, Zoe, Collin, Mark]
- Tested competency of the prepared DH5 [Maddie, Zoe, Mark]
- Prepared three separate overnight cultures of MHD42 cells with parts BBa_B0034, BBa_R0010, and BBa_K1499200 [Alex, Zoe]
Outside Work / Discussion
- Attended Energy, Environmental, and Chemical Engineering (EECE) summer interns orientation [Collin, Mark, Maddie, Zoe, Alex, Micah]
- Discussed each member's designated role and plans for the future
- Made a team calendar
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