In this modified bacteria, the aiiA gene (green rectangle) codifies to our antidote protein (green oval) in an unregulated manner. After some time, the protein production will reach a constant production big enough to act on the quorum sensing regulation. With a constitutive aiiA expression, we expect a complete and uninterrupted inhibition of the AHLs activity by hydrolyzing them and varying the lengths of their acyl chains, modifying their conformational structure. Therefore its autoregulation will be unable to be completed, resulting in no more AHL production.

To model the introduction of this gene into Erwinia amylovora, we first came to several assumptions discussed below.