Difference between revisions of "Team:Aix-Marseille/Safety"

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==Safety==
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<h1> Safety </h1>
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===Safe lab work===
<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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Concerning the safety in the lab, we all had a lecture by Chantal SOSCIA, the prevention officer, about the risks and precautions. This lecture was prior to the wet lab beginning. She told us about basic lab rules (for instance, washing hands before and after an experiment) and basic precautions for our safety: wearing a cotton lab coat, gloves, glasses (if needed), long pants, closed shoes, to have long hair tied. Then she told us about biological and chemical risks, to be aware of the risks before starting an experiment to take the right precautions, how to store the different products. She taught us the risks of several possible cases (like liquid nitrogen, sonicator, etc.) and what precautions to take in each case. And also, the disposal procedures of chemical and biological wastes. And at the end, she gave us a booklet summarizing everything she told us.
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In case of emergency, we had a lifeguard in the lab next door. And he showed us where the extinguishers were.
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We were particularly careful when using toxic chemical compound:
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- GelRed for agarose gel revelation: we were wearing a glove on the hand touching the contaminated things (spatula, gel, revealing box).
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- Chloroform: handled under the fuming hood.
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The experiments on Pseudomonas aeruginosa where carried out by Gauthier DANGLA-PELISSIER, a PhD student, in a level 2 lab, respecting the rules fit for this kind of lab.
  
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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===Safe project design===
 
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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</div>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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===Safe shipment===
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Concerning the shipment of the parts for the Registry, there was no biological issues since the DNA is dry and there are no bacteria. We just followed the protocol and packed everything in a box.
  
 
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Revision as of 15:51, 26 October 2017

Safety

==Safety== bla bla bla ===Safe lab work=== Concerning the safety in the lab, we all had a lecture by Chantal SOSCIA, the prevention officer, about the risks and precautions. This lecture was prior to the wet lab beginning. She told us about basic lab rules (for instance, washing hands before and after an experiment) and basic precautions for our safety: wearing a cotton lab coat, gloves, glasses (if needed), long pants, closed shoes, to have long hair tied. Then she told us about biological and chemical risks, to be aware of the risks before starting an experiment to take the right precautions, how to store the different products. She taught us the risks of several possible cases (like liquid nitrogen, sonicator, etc.) and what precautions to take in each case. And also, the disposal procedures of chemical and biological wastes. And at the end, she gave us a booklet summarizing everything she told us. In case of emergency, we had a lifeguard in the lab next door. And he showed us where the extinguishers were. We were particularly careful when using toxic chemical compound: - GelRed for agarose gel revelation: we were wearing a glove on the hand touching the contaminated things (spatula, gel, revealing box). - Chloroform: handled under the fuming hood. The experiments on Pseudomonas aeruginosa where carried out by Gauthier DANGLA-PELISSIER, a PhD student, in a level 2 lab, respecting the rules fit for this kind of lab. ===Safe project design=== bla bla bla ===Safe shipment=== Concerning the shipment of the parts for the Registry, there was no biological issues since the DNA is dry and there are no bacteria. We just followed the protocol and packed everything in a box.