Difference between revisions of "Team:Worldshaper-XSHS/Model"

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                         <h2>hhhhhhhh</h2>
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                         <h2>Model</h2>
 
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                    <p >
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                        <span style="font-size:10.5000pt;">According to the functional analysis results,</span>
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                        <span>
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<p ><span style="font-size:10.5000pt;">According to the functional analysis results, </span><span>we knew that the fluorescence expressi0n of oxy1 and oxy4 were different.Compared with oxy1, through T7RNA polymerase and T7 &nbsp;promoter, oxy4 will amplify the signal(figure 1).But just how much the signal is amplified, and what is the relationship between it and the dissolved oxygen concentraion, we still don't know.</span><span style="font-size:10.5000pt;">&nbsp;So we explored the model of the correlation between fluorescence expressi0n and oxygen concentration. </span><span  >In modeling, we need to use a lot of knowledge of advanced mathematics. As a senior high school student, we can't use it very well, so we only have a very simple model.But we've really worked very hard.</span><span style="font-size:10.5000pt;">&nbsp;The results are as follows.</span></p>
+
                            we knew that the fluorescence expressi0n of oxy1 and oxy4 were different.Compared with oxy1, through T7RNA polymerase and T7 &nbsp;promoter, oxy4 will amplify the signal(figure 1).But just how much the signal is amplified, and what is the relationship between it and the dissolved oxygen concentraion, we still don't know.
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<p style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BE8.tmp.jpg" alt="" width="419" height="249" /><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                        </span>
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<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure1. The working mechanism of Oxy4</span></p>
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                        <span style="font-size:10.5000pt;">
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<p ><b><span>Oxy1</span></b></p>
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                            &nbsp;So we explored the model of the correlation between fluorescence expressi0n and oxygen concentration.
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">Oxy1-sealed, 475 nm exciting light</span></b></p>
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                        </span>
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<p ><span style="font-size:10.5000pt;">We studied the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration under the condition of sealing, and did the function fitting.</span></p>
+
                        <span  >
−
<p ><span style="position:absolute;z-index:1;left:0px;margin-left:126.7333px;margin-top:99.0000px;width:224.0000px;height:24.0000px;"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BE9.tmp.png" alt="" width="224" height="24" /></span><span style="font-size:10.5000pt;">To make the data compa</span><span style="font-size:10.5000pt;">rable,we standardized the fluorescence intensity and OD600 of each group.</span><span class="15" >&nbsp;Firstly, </span><span style="font-size:10.5000pt;">we su</span><span style="font-size:10.5000pt;">btracted the initial fluorescence intensity and the initial OD600 from the fluorescence intensity and the OD600. </span><span  >Then we divided the fluorescence intensity by the OD600.We had the function fitting for the obtained values, and we got the following functions and images(figure2):</span></p>
+
                            In modeling, we need to use a lot of knowledge of advanced mathematics. As a senior high school student, we can't use it very well, so we only have a very simple model.But we've really worked very hard.
−
<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">&nbsp;r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.87</span></p>
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                        </span>
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<p style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEA.tmp.jpg" alt="" width="540" height="234" /><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                        <span style="font-size:10.5000pt;">&nbsp;The results are as follows.</span>
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<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure2. </span><span style="font-size:9.0000pt;">the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration</span></p>
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                    </p>
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                    <p style="text-align:center;" align="center">
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                        <img src="https://static.igem.org/mediawiki/2017/3/39/T-worldshaper-XSHS-s0001.png" alt="" width="419" height="249" />
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<p ><span style="position:absolute;z-index:1;left:0px;margin-left:145.8667px;margin-top:38.7333px;width:210.0000px;height:24.0000px;"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEB.tmp.png" alt="" width="210" height="24" /></span><span style="font-size:10.5000pt;">When we averaged the values of each group, we got better fitting functions and images(figure3).</span></p>
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<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">&nbsp;r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.87, </span></p>
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                    </p>
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<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEC.tmp.jpg" alt="" width="536" height="226" /><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                    <p  style="text-align:center;" align="center">
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<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure3. </span><span style="font-size:9.0000pt;">the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration</span></p>
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                        <span >figure1. The working mechanism of Oxy4</span>
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                    </p>
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                    <p > <b><span>Oxy1</span></b>  
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<p ><b><span style="font-weight:bold;font-size:11.0000pt;">Oxy1-sealed, 395 nm exciting light</span></b></p>
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                    </p>
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<p ><span style="position:absolute;z-index:1;left:0px;margin-left:139.1333px;margin-top:39.5333px;width:215.0000px;height:24.0000px;"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BED.tmp.png" alt="" width="215" height="24" /></span><span style="font-size:10.5000pt;">For the fluorescence intensity of the excitation light of 395nm, we processed the data in the same way. Here are the functions and images(figure4):</span></p>
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                    <p >
−
<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.99</span></p>
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                        <b><span style="font-weight:bold;font-size:11.0000pt;">Oxy1-sealed, 475 nm exciting light</span></b>  
−
<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEE.tmp.jpg" alt="" width="532" height="230" /><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                    </p>
−
<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure4. </span><span style="font-size:9.0000pt;">the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration</span></p>
+
                    <p>
−
<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                        We studied the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration under the condition of sealing, and did the function fitting.
−
<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                    </p>
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<p ><b><span style="font-weight:bold;font-size:14.0000pt;">Oxy4</span></b></p>
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                    <p>
−
<p ><span style="font-size:10.5000pt;">We also studied the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration under different conditions, and did the function fitting. Data processing was the same as before.</span></p>
+
                        To make the data comparable,we standardized the fluorescence intensity and OD600 of each group.?Firstly, we subtracted the initial fluorescence intensity and the initial OD600 from the fluorescence intensity and the OD600. Then we divided the fluorescence intensity by the OD600.We had the function fitting for the obtained values, and we got the following functions and images(figure2):
−
<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                    </p>
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">Oxy4-sealed, 475 nm exciting light</span></b></p>
+
                    <p >
−
<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.91, </span><span><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEF.tmp.png" alt="" width="145" height="25" /></span></p>
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                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
−
<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BF0.tmp.jpg" alt="" width="528" height="222" /><span style="font-size:9.0000pt;">&nbsp;</span></p>
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                        <span style="font-size:10.5000pt;">&nbsp;r</span> <sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup>  
−
<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure5. </span><span style="font-size:9.0000pt;">the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration</span></p>
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                        <span style="font-size:10.5000pt;">=0.87</span>
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                    </p>
−
<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                    <p style="text-align:center;" align="center">
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">Oxy4-sealed, 395 nm exciting light</span></b></p>
+
                        <img src="https://static.igem.org/mediawiki/2017/7/77/T-worldshaper-XSHS-s0002.png" alt="" width="540" height="234" />
−
<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.88, </span><span><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BF1.tmp.png" alt="" width="137" height="25" /></span></p>
+
                    </p>
−
<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C02.tmp.jpg" alt="" width="556" height="235" /><span style="font-size:9.0000pt;">&nbsp;</span></p>
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                    <p  style="text-align:center;" align="center">
−
<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure6. </span><span style="font-size:9.0000pt;">the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration</span></p>
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                        <span >figure2.</span>
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                        <span >
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<p ><b><span style="font-weight:bold;font-size:11.0000pt;">Oxy4-unsealed, 475 nm exciting light</span></b></p>
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                            the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration
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<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.94, </span><span><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C03.tmp.png" alt="" width="146" height="25" /></span></p>
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                        </span>
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<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C04.tmp.jpg" alt="" width="575" height="237" /><span style="font-size:9.0000pt;">&nbsp;</span></p>
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                    </p>
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<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure7. </span><span style="font-size:9.0000pt;">the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration</span></p>
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                    <p >
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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−
<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
+
                    </p>
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">Oxy4-unsealed, 395 nm exciting light</span></b></p>
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                    <p >
−
<p ><span style="font-size:10.5000pt;">Equation: &nbsp;</span><span style="font-size:10.5000pt;">r</span><sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup><span style="font-size:10.5000pt;">=0.84, </span><span><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C14.tmp.png" alt="" width="135" height="25" /></span></p>
+
                       
−
<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C15.tmp.jpg" alt="" width="599" height="254" /><span style="font-size:9.0000pt;">&nbsp;</span></p>
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                    </p>
−
<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure8. </span><span style="font-size:9.0000pt;">the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration</span></p>
+
                    <p >
−
<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                        <span style="position:absolute;z-index:1;left:0px;margin-left:145.8667px;margin-top:38.7333px;width:210.0000px;height:24.0000px;">
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<p ><span style="font-size:10.5000pt;">&nbsp;</span></p>
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                            <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEB.tmp.png" alt="" width="210" height="24" />
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<p ><b><span style="font-weight:bold;font-size:14.0000pt;">The result of integration</span></b></p>
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                        </span>
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<p ><span  >We integrated the expressi0n models of the two strains.It can be seen that, c</span><span style="font-size:10.5000pt;">ompared with the Oxy1 strain, the fluorescence intensity of the Oxy4 strain was stronger in low oxygen condition, which suggested that the Oxy4 is more suitable for oxygen detection.</span></p>
+
                        <span style="font-size:10.5000pt;">
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<p ><b><span style="font-weight:bold;font-size:11.0000pt;">&nbsp;</span></b></p>
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                            When we averaged the values of each group, we got better fitting functions and images(figure3).
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">integrared, 475 nm exciting light</span></b></p>
+
                        </span>
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<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C16.tmp.png" alt="" width="601" height="304" /><span style="font-size:9.0000pt;">&nbsp;</span></p>
+
                    </p>
−
<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure9. the integrated result of </span><span style="font-size:9.0000pt;">the relationship between fluorescence expressi0n and oxygen concentration</span></p>
+
                    <p >
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">&nbsp;</span></b></p>
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                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
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<p ><b><span style="font-weight:bold;font-size:11.0000pt;">&nbsp;</span></b></p>
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                        <span style="font-size:10.5000pt;">&nbsp;r</span> <sup><span style="font-size:10.5000pt;vertical-align:super;">2</span></sup>  
−
<p ><b><span style="font-weight:bold;font-size:11.0000pt;">integrared, 395 nm exciting light</span></b></p>
+
                        <span style="font-size:10.5000pt;">=0.87,</span>
−
<p  style="text-align:center;" align="center"><img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C17.tmp.png" alt="" width="553" height="280" /><span style="font-size:9.0000pt;">&nbsp;</span></p>
+
                    </p>
−
<p  style="text-align:center;" align="center"><span style="font-size:9.0000pt;">figure10. the integrated result of </span><span style="font-size:9.0000pt;">the relationship between fluorescence expressi0n and oxygen concentration</span></p>
+
                    <p  style="text-align:center;" align="center">
−
 
+
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEC.tmp.jpg" alt="" width="536" height="226" />
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+
                       
−
 
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                    </p>
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                    <p  style="text-align:center;" align="center">
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                        <span >figure3.</span>
−
 
+
                        <span >
−
 
+
                            the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration
−
 
+
                        </span>
−
 
+
                    </p>
−
 
+
                    <p >
−
 
+
                       
−
 
+
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">Oxy1-sealed, 395 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span style="position:absolute;z-index:1;left:0px;margin-left:139.1333px;margin-top:39.5333px;width:215.0000px;height:24.0000px;">
 +
                            <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BED.tmp.png" alt="" width="215" height="24" />
 +
                        </span>
 +
                        <span style="font-size:10.5000pt;">
 +
                            For the fluorescence intensity of the excitation light of 395nm, we processed the data in the same way. Here are the functions and images(figure4):
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
 +
                        <span style="font-size:10.5000pt;">r</span>
 +
                        <sup>
 +
                            <span style="font-size:10.5000pt;vertical-align:super;">2</span>
 +
                        </sup>
 +
                        <span style="font-size:10.5000pt;">=0.99</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEE.tmp.jpg" alt="" width="532" height="230" />
 +
                       
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure4.</span>
 +
                        <span >
 +
                            the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:14.0000pt;">Oxy4</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span style="font-size:10.5000pt;">
 +
                            We also studied the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration under different conditions, and did the function fitting. Data processing was the same as before.
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">Oxy4-sealed, 475 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
 +
                        <span style="font-size:10.5000pt;">r</span>
 +
                        <sup>
 +
                            <span style="font-size:10.5000pt;vertical-align:super;">2</span>
 +
                        </sup>
 +
                        <span style="font-size:10.5000pt;">=0.91,</span>
 +
                        <span>
 +
                            <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BEF.tmp.png" alt="" width="145" height="25" />
 +
                        </span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BF0.tmp.jpg" alt="" width="528" height="222" />
 +
                        <span >&nbsp;</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure5.</span>
 +
                        <span >
 +
                            the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">Oxy4-sealed, 395 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
 +
                        <span style="font-size:10.5000pt;">r</span>
 +
                        <sup>
 +
                            <span style="font-size:10.5000pt;vertical-align:super;">2</span>
 +
                        </sup>
 +
                        <span style="font-size:10.5000pt;">=0.88,</span>
 +
                        <span>
 +
                            <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2BF1.tmp.png" alt="" width="137" height="25" />
 +
                        </span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C02.tmp.jpg" alt="" width="556" height="235" />
 +
                        <span >&nbsp;</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure6.</span>
 +
                        <span >
 +
                            the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">Oxy4-unsealed, 475 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
 +
                        <span style="font-size:10.5000pt;">r</span>
 +
                        <sup>
 +
                            <span style="font-size:10.5000pt;vertical-align:super;">2</span>
 +
                        </sup>
 +
                        <span style="font-size:10.5000pt;">=0.94,</span>
 +
                        <span>
 +
                            <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C03.tmp.png" alt="" width="146" height="25" />
 +
                        </span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C04.tmp.jpg" alt="" width="575" height="237" />
 +
                        <span >&nbsp;</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure7.</span>
 +
                        <span >
 +
                            the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">Oxy4-unsealed, 395 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span style="font-size:10.5000pt;">Equation: &nbsp;</span>
 +
                        <span style="font-size:10.5000pt;">r</span>
 +
                        <sup>
 +
                            <span style="font-size:10.5000pt;vertical-align:super;">2</span>
 +
                        </sup>
 +
                        <span style="font-size:10.5000pt;">=0.84,</span>
 +
                        <span>
 +
                            <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C14.tmp.png" alt="" width="135" height="25" />
 +
                        </span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C15.tmp.jpg" alt="" width="599" height="254" />
 +
                        <span >&nbsp;</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure8.</span>
 +
                        <span >
 +
                            the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                       
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:14.0000pt;">The result of integration</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <span  >
 +
                            We integrated the expressi0n models of the two strains.It can be seen that, c
 +
                        </span>
 +
                        <span style="font-size:10.5000pt;">
 +
                            ompared with the Oxy1 strain, the fluorescence intensity of the Oxy4 strain was stronger in low oxygen condition, which suggested that the Oxy4 is more suitable for oxygen detection.
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">&nbsp;</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">integrared, 475 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C16.tmp.png" alt="" width="601" height="304" />
 +
                        <span >&nbsp;</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure9. the integrated result of</span>
 +
                        <span >
 +
                            the relationship between fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">&nbsp;</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">&nbsp;</span>
 +
                        </b>
 +
                    </p>
 +
                    <p >
 +
                        <b>
 +
                            <span style="font-weight:bold;font-size:11.0000pt;">integrared, 395 nm exciting light</span>
 +
                        </b>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <img src="file:///C:\Users\keywin\AppData\Local\Temp\ksohtml\wps2C17.tmp.png" alt="" width="553" height="280" />
 +
                        <span >&nbsp;</span>
 +
                    </p>
 +
                    <p  style="text-align:center;" align="center">
 +
                        <span >figure10. the integrated result of</span>
 +
                        <span >
 +
                            the relationship between fluorescence expressi0n and oxygen concentration
 +
                        </span>
 +
                    </p>
  
 
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Revision as of 10:40, 29 October 2017

Model

According to the functional analysis results, we knew that the fluorescence expressi0n of oxy1 and oxy4 were different.Compared with oxy1, through T7RNA polymerase and T7  promoter, oxy4 will amplify the signal(figure 1).But just how much the signal is amplified, and what is the relationship between it and the dissolved oxygen concentraion, we still don't know.  So we explored the model of the correlation between fluorescence expressi0n and oxygen concentration. In modeling, we need to use a lot of knowledge of advanced mathematics. As a senior high school student, we can't use it very well, so we only have a very simple model.But we've really worked very hard.  The results are as follows.

figure1. The working mechanism of Oxy4

Oxy1

Oxy1-sealed, 475 nm exciting light

We studied the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration under the condition of sealing, and did the function fitting.

To make the data comparable,we standardized the fluorescence intensity and OD600 of each group.?Firstly, we subtracted the initial fluorescence intensity and the initial OD600 from the fluorescence intensity and the OD600. Then we divided the fluorescence intensity by the OD600.We had the function fitting for the obtained values, and we got the following functions and images(figure2):

Equation:    r 2 =0.87

figure2. the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration

When we averaged the values of each group, we got better fitting functions and images(figure3).

Equation:    r 2 =0.87,

figure3. the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration

Oxy1-sealed, 395 nm exciting light

For the fluorescence intensity of the excitation light of 395nm, we processed the data in the same way. Here are the functions and images(figure4):

Equation:   r 2 =0.99

figure4. the relationship between Oxy1 bacterial fluorescence expressi0n and oxygen concentration

Oxy4

We also studied the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration under different conditions, and did the function fitting. Data processing was the same as before.

Oxy4-sealed, 475 nm exciting light

Equation:   r 2 =0.91,

 

figure5. the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration

Oxy4-sealed, 395 nm exciting light

Equation:   r 2 =0.88,

 

figure6. the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration

Oxy4-unsealed, 475 nm exciting light

Equation:   r 2 =0.94,

 

figure7. the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration

Oxy4-unsealed, 395 nm exciting light

Equation:   r 2 =0.84,

 

figure8. the relationship between Oxy4 bacterial fluorescence expressi0n and oxygen concentration

The result of integration

We integrated the expressi0n models of the two strains.It can be seen that, c ompared with the Oxy1 strain, the fluorescence intensity of the Oxy4 strain was stronger in low oxygen condition, which suggested that the Oxy4 is more suitable for oxygen detection.

 

integrared, 475 nm exciting light

 

figure9. the integrated result of the relationship between fluorescence expressi0n and oxygen concentration

 

 

integrared, 395 nm exciting light

 

figure10. the integrated result of the relationship between fluorescence expressi0n and oxygen concentration

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