Difference between revisions of "Team:Stony Brook/Experiments"
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<h3> Nanodrop Protocol </h3> | <h3> Nanodrop Protocol </h3> | ||
<p> | <p> | ||
| − | Select Nucleic Acid | + | Select Nucleic Acid</p> |
| − | < | + | <p>Clean pedestal, drop 1ul water, press okay</p> |
| − | Clean pedestal, drop 1ul water, press okay | + | <p>Blank with 1ul of room temperature water</p> |
| − | < | + | <p>Measure with 1ul of DNA</p> |
| − | Blank with 1ul of room temperature water | + | <p>260/280 ratio should be near 2 |
| − | < | + | |
| − | Measure with 1ul of DNA | + | |
| − | + | ||
| − | 260/280 ratio should be near 2 | + | |
</p> | </p> | ||
</section> | </section> | ||
Revision as of 20:49, 29 October 2017
Protocols and Experiments
Luria Bertani Broth Protocol
This protocol makes 1L of LB and can be adjusted as needed.
Combine and mix using a magnetic stir bar on low heat. Autoclave for 20 minutes on a liquid cycle.
Luria Bertani Agar Plate Protocol
Combine and mix using a magnetic stir bar on low heat. Autoclave for 20 minutes on a liquid cycle. Allow LB agar to cool to about 55 oC before adding antibiotic. Pour into plates.
Nanodrop Protocol
Select Nucleic Acid
Clean pedestal, drop 1ul water, press okay
Blank with 1ul of room temperature water
Measure with 1ul of DNA
260/280 ratio should be near 2
