Difference between revisions of "Team:NCKU Tainan/Contribution"
Ssccb12040 (Talk | contribs) |
Ssccb12040 (Talk | contribs) |
||
| Line 72: | Line 72: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 454. | + | 454.6 |
</td> | </td> | ||
<td> | <td> | ||
| − | 546. | + | 546.8 |
</td> | </td> | ||
<td> | <td> | ||
| − | 620. | + | 620.4 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 86: | Line 86: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 689. | + | 689.9 |
</td> | </td> | ||
<td> | <td> | ||
| − | 754. | + | 754.0 |
</td> | </td> | ||
<td> | <td> | ||
| − | 821. | + | 821.1 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 100: | Line 100: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 766. | + | 766.8 |
</td> | </td> | ||
<td> | <td> | ||
| − | 866. | + | 866.8 |
</td> | </td> | ||
<td> | <td> | ||
| − | 922. | + | 922.8 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 114: | Line 114: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 816. | + | 816.4 |
</td> | </td> | ||
<td> | <td> | ||
| − | 916. | + | 916.1 |
</td> | </td> | ||
<td> | <td> | ||
| − | 972. | + | 972.6 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 128: | Line 128: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 831. | + | 831.8 |
</td> | </td> | ||
<td> | <td> | ||
| − | 931. | + | 931.4 |
</td> | </td> | ||
<td> | <td> | ||
| − | 985. | + | 985.1 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 142: | Line 142: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 900. | + | 900.1 |
</td> | </td> | ||
<td> | <td> | ||
| − | 998. | + | 998.9 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1058. | + | 1058.8 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 156: | Line 156: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 962. | + | 962.4 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1093. | + | 1093.0 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1148. | + | 1148.2 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 170: | Line 170: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 1090. | + | 1090.9 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1200. | + | 1200.9 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1252. | + | 1252.1 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 184: | Line 184: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 1129. | + | 1129.6 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1253. | + | 1253.0 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1295. | + | 1295.0 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 198: | Line 198: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 1240. | + | 1240.3 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1341. | + | 1341.7 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1373. | + | 1373.6 |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 212: | Line 212: | ||
</td> | </td> | ||
<td> | <td> | ||
| − | 1259. | + | 1259.7 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1385. | + | 1385.9 |
</td> | </td> | ||
<td> | <td> | ||
| − | 1449. | + | 1449.9 |
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 12:27, 30 October 2017
Contribution
Introduction
Nitrate is toxic to fish or shrimp in water. When nitrate reach a certain concentration level, aquatic animal will start to die. So, in order to maintain good quality of water, concentration of nitrate in water need to be tested every day. In corporate to our team’s project “NO PROBLEM” this year, decide to sense the concentration of nitrite and nitrate as a part of our project.
Description
Part: BBa_K381001 was first designed by Katharine Coyte from team BCCS-Bristol in iGEM 2010. It is a Nitrate reporter, PyeaR - GFP composite. The team BCCS-Bristol only test the BioBrick’s sensitivity at a higher concentration, but the Nitrate level of aquatic water usually won’t reach 1 mM. Therefore, we carry out experiment by testing the fluorescence intensity with ppm level of Potassium Nitrate, which is much lower than all the teams before. The results are shown below.
Figure 1. The fluorescence intensity of different Nitrate concentration taken the logarithm at 8, 12 and 24 hours.
It shows that the BioBrick BBa_K381001 have nice sensitivity not just in high Nitrate concentration, but low as well. Many teams had done improvements of K381001, but our team bring out the most beautiful data. And we also take X axis logarithm, which can make our data become linear. Not only easier to read, but also more meaningful.
The data is shown as below.
| ppm conc | 8 hours | 12 hours | 24 hours |
|---|---|---|---|
| 0 | 454.6 | 546.8 | 620.4 |
| 2 | 689.9 | 754.0 | 821.1 |
| 4 | 766.8 | 866.8 | 922.8 |
| 6 | 816.4 | 916.1 | 972.6 |
| 8 | 831.8 | 931.4 | 985.1 |
| 10 | 900.1 | 998.9 | 1058.8 |
| 20 | 962.4 | 1093.0 | 1148.2 |
| 40 | 1090.9 | 1200.9 | 1252.1 |
| 60 | 1129.6 | 1253.0 | 1295.0 |
| 80 | 1240.3 | 1341.7 | 1373.6 |
| 100 | 1259.7 | 1385.9 | 1449.9 |
