Difference between revisions of "Team:SCU China/HP/Gold Integrated"

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<h3>★  ALERT! </h3>
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                <!-- start of content -->
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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                <div class="content_wrapper">
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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                    <div class="scu_detail_wrapper">
</div>
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                        <div class="lee_nav_image">
<div class="clear"></div>
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                            <img class="scu_nav_project_black"
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                                src="https://static.igem.org/mediawiki/2017/2/27/T--SCU_China--bannerBlack.png">
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                            <img class="scu_nav_background"
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                                src="https://static.igem.org/mediawiki/2017/d/d8/T--SCU_China--bannerBack.png"
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                                id="scu_nav_bannerBack">
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                        </div>
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                                              <div class="nav nav-justified scu_nav" id="scu_welcome">
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                                                        <li class="lee_nav_home">
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                                <a href="https://2017.igem.org/Team:SCU_China">Home</a>
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                            </li>
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                            <li class="dropdown lee_nav_project">
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                                <a class="dropdown-toggle" data-toggle="dropdown" href="">
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                                    Project
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                                </a>
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                                <ul class="dropdown-menu">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Repressilator">Repressilator</a>
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                                    </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Malatonion">Melatonin</a>
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                                    </li>
  
<div class="column full_size">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/InterLab">InterLab</a></li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Parts">Parts</a></li>
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                                </ul>
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                            </li>
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                            <li class="dropdown lee_nav_modeling">
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                                <a href="https://2017.igem.org/Team:SCU_China/Model" class="dropdown-toggle" data-toggle="dropdown">
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                                    Modeling
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                                </a>
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                                <ul class="dropdown-menu">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Model">Overview</a>
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                                    </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Model/Repressilator">Repressilator</a>
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                                    </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Model/Melatonin">Melatonin</a>
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                                    </li>
  
<h1>Gold Medal and Integrated Human Practices</h1>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Model/RBSModification">RBS Modification</a>
  
<p>This page will contain information for your Gold medal Human Practices work, which you can also use to nominate your team for the Best Integrated Human Practices page. To make things easier, we have combined the Gold medal page with the Best Integrated Human Practices page since we expect the work to overlap considerably. </p>
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                                    </li>
<p>iGEM teams are unique and leading the field because they "go beyond the lab" to imagine their projects in a social/environmental context, to better understand issues that might influence the design and use of their technologies.</p>
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                                </ul>
<p>Teams work with students and advisors from the humanities and social sciences to explore topics concerning ethical, legal, social, economic, safety or security issues related to their work. Consideration of these Human Practices is crucial for building safe and sustainable projects that serve the public interest. </p>
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                            </li>
<p>For more information, please see the <a href="https://2017.igem.org/Competition/Human_Practices">Human Practices page</a>.</p>
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                            <li class="dropdown lee_nav_human">
</div>
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                                <a href="https://2017.igem.org/Team:SCU_China/HP/Silver" class="dropdown-toggle"
<div class="clear"></div>
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                                  data-toggle="dropdown">Human Practices
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                                </a>
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                                <ul class="dropdown-menu">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver">Overview</a>
  
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                                    </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Survey">Survey</a>
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                                    </li>
  
<div class="column half_size">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Meetup">Meet-up</a>
<h3>Gold Medal Criterion #1</h3>
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                                    </li>
<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.</p>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Engagement">Engagement</a>
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                                    </li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Collaborations">Collaboration</a>
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                                    </li>
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                                </ul>
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                            </li>
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                            <li class="dropdown lee_nav_attri">
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                                <a href="https://2017.igem.org/Team:SCU_China/Attributions">
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                                    Attribution
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                                </a>
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                            </li>
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                            <li class="dropdown lee_nav_gold">
  
</div>
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                                <a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated" class="dropdown-toggle" data-toggle="dropdown">
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                                    Gold Medal
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                                </a>
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                                <ul class="dropdown-menu">
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated">Integrated HP</a></li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/HP/Improve">Improve</a></li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Model">Model</a></li>
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                                    <li><a href="https://2017.igem.org/Team:SCU_China/Demonstrate">Demonstrate</a></li>
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                                </ul>
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                            </li>
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                            <li class="lee_nav_note">
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                                <a href="https://2017.igem.org/Team:SCU_China/Notebook">
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                                    Notebook
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                                </a>
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                            </li>
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                        </div>
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                        <div class="scu_nav_side" id="scu_nav_side">
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                            <!--<div class="scu_nav_side_image">-->
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                            <!--<img src="https://static.igem.org/mediawiki/2017/b/b6/T&#45;&#45;SCU_China&#45;&#45;nav-side.png">-->
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                            <!--</div>-->
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                            <div id="nav_tree">
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                                <ul class="scu_nav_tree">
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                                    <li>Integrated <br>Human Practice
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                                        <ul>
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                                            <li><a href="#determination">Project<br>Determination</a></li>
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                                            <li><a href="#improvement">Project<br>Improvement</a></li>
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                                            <li><a href="#experiments">Experiments</a></li>
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                                        </ul>
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                                    </li>
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                                </ul>
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                            </div>
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                        </div>
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                        <div class="scu_content_wrapper">
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                            <div class="overview-title scu_detail_title" id="determination">Integrated Human Practice
 +
                            </div>
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                            <div class="scu_split2" id="community_class"></div>
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                            <div class="scu_sub_wrapper">
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                                <p class="scu_content_p">In this year, human practices were integrated to the whole
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                                    process of our project as the following flowchart (Fig.1).
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                                </p>
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                                <div>
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                                    <img class="scu_hp_image_single"
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                                        src="https://static.igem.org/mediawiki/2017/f/f2/T-SCU_China_2017-integrated_HP_fig1.png">
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                                    <p class="scu_img_index"> Fig.1 </p>
 +
                                </div>
 +
                                <div class="scu_sub_title"><i>01.</i>&nbsp;&nbsp;Project Determination</div>
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                                <p class="scu_content_p">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Our team has always focused
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                                    on
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                                    solving the existing issue seen in the society, and we value our connection with the
 +
                                    vast public as well as our counterparts. For the year of 2017, we picked up two
 +
                                    candidate projects – both of which were trying to provide possible solutions to
 +
                                    present-day issues – one was “Rabies Vaccine Taken by Mouth” and the other was
 +
                                    “Rhythmic Production of Melatonin in E. coli”. We always want to be of help of our
 +
                                    world; so before making the final choice, we looked carefully into the potential
 +
                                    social and scientific value of both projects, by means of initiating field survey,
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                                    interview professors and experts from universities and authorities as well as
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                                    collaborating with other iGEM teams.<br>
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                                    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Our team has always focused on solving the
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                                    existing
 +
                                    issue seen in the society, and
 +
                                    we value our connection with the vast public as well as our counterparts. For the
 +
                                    year of 2017, we picked up two candidate projects – both of which were trying to
 +
                                    provide possible solutions to present-day issues – one was “Rabies Vaccine Taken by
 +
                                    Mouth” and the other was “Rhythmic Production of Melatonin in E. coli”. We always
 +
                                    want to be of help of our world; so before making the final choice, we looked
 +
                                    carefully into the potential social and scientific value of both projects, by means
 +
                                    of initiating field survey, interview professors and experts from universities and
 +
                                    authorities as well as collaborating with other iGEM teams.<br>
 +
                                                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We initiated <a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Survey#questionnairs">a field survey </a>on the sleeping
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                                    quality and solutions Human Practice-Survey-Questionnairs to sleeping issues on the
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                                    streets and via the Web. The questionnaire used in the survey contains questions
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                                    considering sleeping quality of both groups having regular timetable and working at
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                                    night, respectively, and the questions on solving sleeping problems and views on
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                                    using medicine to improve sleeping quality. The result shows that a large portion of
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                                    interviewees had sleeping problems, some of which suffered from severe symptoms;
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                                    however, few figured out a way to tackle with the issues. Meanwhile, when it comes
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                                    to the medicine, basic acknowledgement of traditional benzodiazepines medicines was
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                                    observed, but seldom do people know about the medicine of melatonin.<br>
  
<div class="column half_size">
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                                    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To conclude, according to the survey, we regard
<h3>Best Integrated Human Practices Special Prize</h3>
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                                    it
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                                    very meaningful and prospective
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                                    to employ the synthetic biology approaches to present new solutions to the issues
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                                    considering sleeping. With this knowledge as assurance, we began our design on the
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                                    project tackling sleeping problems.<br>
  
<p>
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                                    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;While we find the project on melatonin more
To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Integrated Human Practices prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>.
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                                    interesting and promising, the<a
<br><br>
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                                            href="https://2017.igem.org/Team:SCU_China/HP/Silver/Meetup#iSWU"> iSWU
You must also delete the message box on the top of this page to be eligible for this prize.
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                                        conference </a>led us to our final
</p>
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                                    decision. iSWU is the organization consisting of all the iGEM team in the southwest
</div>
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                                    of China, providing us the platform to share with each other our projects and
<div class="clear"></div>
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                                    thoughts on synthetic biology. At the conference, all of the teams introduced their
<div class="column full_size">
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                                    project prototype and made comments on others’ designs. At the meeting, a professor
<h5>Inspiration</h5>
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                                    from TMMU with rich experience in immunology suggested that if we do the project
<p>Here are a few examples of excellent Integrated Human Practices work:</p>
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                                    about the rabies vaccine, unpreventable immunology experiments would cost a lot of
<ul>
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                                    time and the results would be highly unpredictable as well – in a word, it would be
<li><a href="https://2016.igem.org/Team:INSA-Lyon/Integrated_Practices">2016 INSA Lyon</a></li>
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                                    very difficult to complete all the experiments within three months’ time. The new
<li><a href="https://2016.igem.org/Team:UofC_Calgary/Integrated_Practices">2016 UofC Calgary</a></li>
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                                    information tilts the balance one step more.<br>
<li><a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Practices">2015 Bielefeld</a></li>
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<li><a href="https://2015.igem.org/Team:Edinburgh/Practices">2015 Edinburgh</a></li>
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                                    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;After carefully evaluating the advice from other
</ul>
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                                    team and comparing the two
 +
                                    projects, we chose “Rhythmic Production of Melatonin in E.coli” because it was more
 +
                                    practical with more predictable result
 +
 
 +
                                </p>
 +
                                <div class="scu_split" id="improvement"></div>
 +
                                <div class="scu_sub_title"><i>02.</i>&nbsp;&nbsp;Improvement</div>
 +
                                <div>
 +
                                    <p class="scu_content_p">
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;After determining the project, we found that
 +
                                        there
 +
                                        were many problems remained to solve. Therefore, we consulted teachers and
 +
                                        experts from Sleep Medical Center, <a
 +
                                            href="https://2017.igem.org/Team:SCU_China/HP/Silver/Survey#sleep_center">West
 +
                                        China Hospital</a> and <a
 +
                                            href="https://2017.igem.org/Team:SCU_China/HP/Silver/Survey#drug_administration">China
 +
                                        Food and Drug Administration (CFDA)</a>.
 +
                                        What’s more, we also
 +
                                        communicated with other iGEM teams via Skype.<br>
 +
 
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;During the interview with the Sleep Medical
 +
                                        Center faculties, the professors
 +
                                        described the mechanism of melatonin in detail as well as melatonin’s advantages
 +
                                        over other drugs, which gave us a whole new view on melatonin. They also
 +
                                        emphasized what to focus during the research, such as the safety issues emerged
 +
                                        when we tried to produce E.coli strains secreting melatonin in human body, the
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                                        dedicate relationship between the circadian rhythm of melatonin production and
 +
                                        sleep related neural circuits and possible test on mouse in vivo. At the same
 +
                                        time, to our delight the professors highly appreciated the practicality and
 +
                                        novelty of our project. During the interview with the experts of CFDA, we got
 +
                                        known of the national standards on melatonin in Mainland China, which drove us
 +
                                        to focus more on legal issues and safety considerations.
 +
                                        <br>
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We had video chat with Team Purdue to
 +
                                        introduce our respective projects. In this
 +
                                        video chat, their real condition simulation experiments inspired us to do some
 +
                                        experiments to simulate growth conditions in the environment of gut. In
 +
                                        addition, we were inspired by team “SCU-WestChina”’s modeling of constitutive
 +
                                        promoter family to make our modeling of enzyme kinetics by ourselves, in order
 +
                                        to achieve more efficient production of melatonin with reasonable formula of
 +
                                        enzymes.
 +
                                        <br>
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;With the help of the Sleep Medical Center,
 +
                                        CFDA and other iGEM teams, we finally
 +
                                        arrived at the complete project design and improvement.
 +
                                    </p>
 +
                                </div>  
 +
                                <div class="scu_split" id="experiments"></div>
 +
                                <div class="scu_sub_title"><i>03.</i>&nbsp;&nbsp;Experiments</div>
 +
                                <div>
 +
                                    <p class="scu_content_p">
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;During the following experiments, we
 +
                                        encountered many problems as well; luckily,
 +
                                        most of them were solved with our toil and others’ assistance.<br>
 +
 
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;SCAU-China provided LUDOX in the measurement
 +
                                        kit, which turned out to be crucial
 +
                                        in our InterLab experiments.<br>
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Limited to the rules of using plate reader
 +
                                        in our public lab, we cannot use the
 +
                                        plate reader in our public lab for continuous kinetic measurement of
 +
                                        repressilator over 8h. Thankfully, Team “SCU-WestChina” provided us plate reader
 +
                                        “Biotek Cytation 3 Cell Imaging Multi-Mode Reader” in their lab to finish such
 +
                                        experiments.<br>
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;During CCiC, we learned that the project of
 +
                                        the iGEM team of Peking University
 +
                                        was closely related to repressilator as well, so we exchanged our experience on
 +
                                        repressilator and maintained constant communication since. After the conference,
 +
                                        we found in our experiment that the plasmid pLPT107 cannot be transformed into
 +
                                        the ΔclpXPMC4100 stain that was knocked out by ourselves; thus, we turned to
 +
                                        team "Peking" immediately. Gratefully, they sent their ΔclpXP strain to us
 +
                                        immediately and shared their experience with us. This helped us a lot in keeping
 +
                                        our schedule.<br>
 +
                                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In all of the above mentioned survey,
 +
                                        interview, study and communication, we
 +
                                        obtained information and assistance that pushed us forward and enabled us to
 +
                                        complete our project step by step. Finally, through our own hard work and the
 +
                                        generous help of others, we finished a thorough, complete project with practical
 +
                                        value.
 +
                                    </p>
 +
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                        <p class="scu_footer_text_2">Contact Us: SCU_China2017@126.com</p>
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Latest revision as of 00:27, 31 October 2017

Integrated Human Practice

In this year, human practices were integrated to the whole process of our project as the following flowchart (Fig.1).

Fig.1

01.  Project Determination

      Our team has always focused on solving the existing issue seen in the society, and we value our connection with the vast public as well as our counterparts. For the year of 2017, we picked up two candidate projects – both of which were trying to provide possible solutions to present-day issues – one was “Rabies Vaccine Taken by Mouth” and the other was “Rhythmic Production of Melatonin in E. coli”. We always want to be of help of our world; so before making the final choice, we looked carefully into the potential social and scientific value of both projects, by means of initiating field survey, interview professors and experts from universities and authorities as well as collaborating with other iGEM teams.
      Our team has always focused on solving the existing issue seen in the society, and we value our connection with the vast public as well as our counterparts. For the year of 2017, we picked up two candidate projects – both of which were trying to provide possible solutions to present-day issues – one was “Rabies Vaccine Taken by Mouth” and the other was “Rhythmic Production of Melatonin in E. coli”. We always want to be of help of our world; so before making the final choice, we looked carefully into the potential social and scientific value of both projects, by means of initiating field survey, interview professors and experts from universities and authorities as well as collaborating with other iGEM teams.
      We initiated a field survey on the sleeping quality and solutions Human Practice-Survey-Questionnairs to sleeping issues on the streets and via the Web. The questionnaire used in the survey contains questions considering sleeping quality of both groups having regular timetable and working at night, respectively, and the questions on solving sleeping problems and views on using medicine to improve sleeping quality. The result shows that a large portion of interviewees had sleeping problems, some of which suffered from severe symptoms; however, few figured out a way to tackle with the issues. Meanwhile, when it comes to the medicine, basic acknowledgement of traditional benzodiazepines medicines was observed, but seldom do people know about the medicine of melatonin.
      To conclude, according to the survey, we regard it very meaningful and prospective to employ the synthetic biology approaches to present new solutions to the issues considering sleeping. With this knowledge as assurance, we began our design on the project tackling sleeping problems.
      While we find the project on melatonin more interesting and promising, the iSWU conference led us to our final decision. iSWU is the organization consisting of all the iGEM team in the southwest of China, providing us the platform to share with each other our projects and thoughts on synthetic biology. At the conference, all of the teams introduced their project prototype and made comments on others’ designs. At the meeting, a professor from TMMU with rich experience in immunology suggested that if we do the project about the rabies vaccine, unpreventable immunology experiments would cost a lot of time and the results would be highly unpredictable as well – in a word, it would be very difficult to complete all the experiments within three months’ time. The new information tilts the balance one step more.
      After carefully evaluating the advice from other team and comparing the two projects, we chose “Rhythmic Production of Melatonin in E.coli” because it was more practical with more predictable result

02.  Improvement

      After determining the project, we found that there were many problems remained to solve. Therefore, we consulted teachers and experts from Sleep Medical Center, West China Hospital and China Food and Drug Administration (CFDA). What’s more, we also communicated with other iGEM teams via Skype.
      During the interview with the Sleep Medical Center faculties, the professors described the mechanism of melatonin in detail as well as melatonin’s advantages over other drugs, which gave us a whole new view on melatonin. They also emphasized what to focus during the research, such as the safety issues emerged when we tried to produce E.coli strains secreting melatonin in human body, the dedicate relationship between the circadian rhythm of melatonin production and sleep related neural circuits and possible test on mouse in vivo. At the same time, to our delight the professors highly appreciated the practicality and novelty of our project. During the interview with the experts of CFDA, we got known of the national standards on melatonin in Mainland China, which drove us to focus more on legal issues and safety considerations.
      We had video chat with Team Purdue to introduce our respective projects. In this video chat, their real condition simulation experiments inspired us to do some experiments to simulate growth conditions in the environment of gut. In addition, we were inspired by team “SCU-WestChina”’s modeling of constitutive promoter family to make our modeling of enzyme kinetics by ourselves, in order to achieve more efficient production of melatonin with reasonable formula of enzymes.
      With the help of the Sleep Medical Center, CFDA and other iGEM teams, we finally arrived at the complete project design and improvement.

03.  Experiments

      During the following experiments, we encountered many problems as well; luckily, most of them were solved with our toil and others’ assistance.
      SCAU-China provided LUDOX in the measurement kit, which turned out to be crucial in our InterLab experiments.
      Limited to the rules of using plate reader in our public lab, we cannot use the plate reader in our public lab for continuous kinetic measurement of repressilator over 8h. Thankfully, Team “SCU-WestChina” provided us plate reader “Biotek Cytation 3 Cell Imaging Multi-Mode Reader” in their lab to finish such experiments.
      During CCiC, we learned that the project of the iGEM team of Peking University was closely related to repressilator as well, so we exchanged our experience on repressilator and maintained constant communication since. After the conference, we found in our experiment that the plasmid pLPT107 cannot be transformed into the ΔclpXPMC4100 stain that was knocked out by ourselves; thus, we turned to team "Peking" immediately. Gratefully, they sent their ΔclpXP strain to us immediately and shared their experience with us. This helped us a lot in keeping our schedule.
      In all of the above mentioned survey, interview, study and communication, we obtained information and assistance that pushed us forward and enabled us to complete our project step by step. Finally, through our own hard work and the generous help of others, we finished a thorough, complete project with practical value.