Team:SCU China/Attributions
Sincere thanks to all those who have helped us over this year, not only for helping on the project, but also for experiencing an unforgettable journey together. Here we describe what we have done by ourselves this year and what was done by others in detail.
We started to prepare for
2017 iGEM a few weeks after the 2016 iGEM. Team leader, vice team leader and group
leaders and reserved team members were determined at that time. We held a short
training lasting for almost one week in winter holiday with the help of our
instructors. Brainstorms were held to come up with our new project during the
training. Several reserved projects were chosen and being considered carefully in
Spring semester. During the Spring semester, we held project analysis for several
times to learn from other teams. ACMA was successfully held as well. General project
frame was determined in May with the help of our teachers, advisors, as well as
previous iGEMers. Experiments were done from July to October.
Details of our winter training will also be introduced
to you here.
Winter training was held a few days after Fall
semester. In a training lasting for
one week, teachers and speakers will present the basic ideas and knowledge related
to molecular biology and synthetic biology. Laboratory safety will also be
emphasized. Our primary PI Dr. Nianhui Zhang will lead students to do some basic
experiments. Timetable of this year’s winter training is shown in figure 1. Students
in winter training mainly are reserved team member selected before. The training is
not obligated to reserved team members. Though our materials are not available to
all students in our college, people who are not in our reserved team but want to
participate the training is welcomed. Training material is totally available to
them, too. Students will be grouped before the training, including people who are
not in our reserved team. Brainstorms were held every night based on groups. On the
last day, we will hold project defense to show our primary ideas about project.
Fig.2
Attributions related to what we mentioned in detail is shown below.
Dr. Nianhui Zhang, team primary PI,
teacher of Molecular
Biology Experiments in winter training, advisor of project defense, judge of ACMA,
provided laboratory for us and pay for expenses on account.
Dr. Chuanfang Wu, team secondary PI, teacher of Molecular Biology
in winter
training, advisor of project defense, judge of ACMA.
Fig.3
Junyan Zhu, team leader of SCU_China, one of the group leader of
melatonin (aimed
to produce melatonin successfully and verify enzymes’ function), speaker of
Synthetic Biology Discussion in winter training, designed the experiments of group
melatonin, expressed four enzymes successfully, wrote related wiki content.
Han Geng, vice team leader of SCU_China, group leader of
represillator (aimed to verify the function of represillator), speaker of Synthetic
Biology Discussion in winter training, lecturer in CCiC, came up with the original
project idea during winter training, measured the fluorescence of repressilator.
Jialu Li, one of the group leader of melatonin, purified four
enzymes successfully, also in charge of the reagent purchase this year.
Xinhao Yan, group leader of knockout (aimed to knockout ClpXP
enzyme), speaker of Synthetic Biology Discussion in winter training, wrote related
wiki content.
Xinpeng Zhang, group leader of modeling, speaker of Synthetic
Biology Discussion in winter training, lecturer in CCiC, managed the modeling in our
project and give advice based on modeling result, wrote related wiki content.
Yiwen Zhao, group leader of quorum sensing (aimed at synchronizing
the oscillated period), designed and constructed quorum sensing circuit, helped in
fluorescent measurement of repressilator, wrote related wiki content.
Tianying Chen, one of the group leader of InterLab (aimed to
complete InterLab measurement and modified RBS), speaker of Synthetic Biology
Discussion in winter training.
Bowen Li, manager of verifying enzyme activities, contacted with
Dr. Hui Jin to get the permission of using HPLC with autosampler in College of
Pharmacy, ran HPLC for verification, wrote related wiki content.
Yi Shan, art designer, designer of team uniform, poster for ACMA
and so on.
Hao Yuan, manager of wiki page, uploaded wiki content, wrote
related code and did typesetting.
Fuqiang Yang, manager of InterLab measurement and did related
experiments, helped in expression of melatonin and verification of enzyme
activities, helped in the survey of human practice, wrote related wiki content.
Wei Lin, did experiments related to construction and verification
of quorum sensing circuit, wrote related wiki content.
Zhiwei Luo, did experiments related to expression of melatonin,
helped in survey in human practice, wrote related part content.
Yibing Tao, did experiments related to construction and
verification of quorum sensing circuit, measured fluorescence of repressilator,
wrote related wiki content.
Xuetong Qu, did experiments related to construction and
verification of quorum sensing circuit, helpd in organization and survey of human
practice.
Haoyang Cheng, did experiments related to expression of melatonin,
checkout wiki content written by others.
Yuliang Xiang, did experiments related to verification of enzyme
activities by HPLC.
Hongjin Lai, did experiments related to construction of quorum
sensing circuit, helped in ClpXP knockout.
Chunyi Yan, did experiments related to ClpXP knockout and helped in
construction of quorum sensing circuit and human practices.
Ziru Wang, did experiments related to ClpXP knockout and helped in
construction and verification of quorum sensing circuit.
Junjie Xu, did experiments related to RBS modification.
Mingyi Lv, did experiments related to RBS modification.
Fig.4
Tianyi Zhang, reversed team member, did fluorescence
measurement for repressilator, participated in formation of project idea.
Caiqin Wang, reversed team member, helped in InterLab study and
human practice.
Yumei Yang, reversed team member, helped in verification of
enzyme activities.
Yuqing Zhao, reversed team member, helped in RBS modification
and human practice, as well as modeling, wrote a part of wiki page about human
practice.
Qinlin Qiu, reversed team member, helped in construction of
quorum sensing circuit and wrote a part of wiki page about human practice.
Tian Liu, reversed team member, helped in InterLab study and
human practice, wrote a part of wiki page about human practice.
Yuanhan Yang, reversed team member, helped in InterLab study
and human practice.
Chenming Zhang, reversed team member, helped in InterLab
measurement and prepared questionnaire of human practice and some other
activities in human practice.
Yanling Zhong, reversed team member, helped in InterLab
study.
Changhe Li, reversed team member, helped in InterLab study.
Linfeng Li, reversed team member, helped in knockout of
ClpX.
Mucun Hou, reversed team member, helped in expressing melatonin
related enzymes.
First of all, we are very grateful to Sichuan University, College of Life Sciences,
Wu Yuzhang Honors College and College of Physical Science and Technology for
supporting us in the 2017 iGEM competition. In particular, we are deeply thankful to
College of Life Sciences, which provides us with the independent laboratories and
laboratory funding.
Secondly, we want to sincerely acknowledge the seniors and teachers of our
university, who encouraged and helped us in different aspects.
Winter Training and ACMA
Dr. Xiaoping Wan, advisor of project defense, offered help in holding ACMA, as well as being one of judge of ACMA.
Dr. Ying Tong, teacher of Commonly Used Experimental Methods in Modern Biology and Case Study in winter training, advisor of project defense, judge of ACMA.
Dr. Jinmin Wu, judge of ACMA.
Dr. Fangdong Zou, judge of ACMA.
Dr. Qun Sun, judge of ACMA.
Project and Experiments
Dr. Fei Huang, advisor of project defense.
Dr. Xiaofeng Zhu, advisor of project defense, judge of ACMA, generously provided extra shaker for us.
Dr. Dong Yu, teacher in charge of public laboratory in College of Life Science, taught us how to use HPLC, offered some bacterial filters and injectors when they were used up.
Dr. Han Kang, teacher in charge of plate reader (ThermoScientific Varioskan Flash), assisted us with our fluorescence measurement and InterLab work, manipulating the instrument.
Dr. Zhonghan Li, offered plated reader when instrument managed by Dr. Han Kang is unavailable (though data was not used).
Dr. Chong Chen, offered plated reader which can be used to measure throughout the whole night.
Dr. Hui Jin, teacher in College of Pharmacy of SCU, permitted us to use HPLC with autosampler in College of Pharmacy.
Dr. Ye Zhang, interviewee of Human Practice, accepted our interview in human practice.
Secondly, we sincerely thank to students and seniors of our university, who provided advice to us.
Qian Jiang, master in College of Pharmacy of SCU, generously offered mice’s feces to us.
Wenjiao Xiao, master in College of Pharmacy of SCU, taught us how to use HPLC with autosampler in College of Pharmacy.
Hongbin Yu, team leader of SCU-WestChina, offered help in optimizing project design, assisted with troubleshooting related to enzyme expression.
Zikuan Song, team member of SCU-WestChina, helped in connecting related officer of Chengdu Library to hold an exhibition.
Jiusi Guo team member of SCU-WestChina, helped in connecting usage of plate reader in Dr. Chong Chen’s lab.
Specially, thanks to team “SCU-WestChina” that helped us in many aspects.
Last but not least, we are deeply grateful to the labs and iGEMers of other schools that have provided us with help.
Thanks to Jianxin Lv and Wu Wang's lab in Wenzhou Medical University that provided us wide type MC4100 strain for our experiments about repressilator.
Thanks to team “Peking” that provided us ΔclpXP MC4100 stain (DHL708) for our experiments about repressilator.
Thanks to team “SiCAU-China” that provided us LUDOX for our InterLab study.
Thanks to team “TMMU-China” that gave us some suggestions for our project determination.
