Difference between revisions of "Team:SCU China/Attributions"

      We started to prepare for 2017 iGEM a few weeks after the 2016 iGEM. Team leader, vice team leader and group leaders and reserved team members were determined at that time. We held a short training lasting for almost one week in winter holiday with the help of our instructors. Brainstorms were held to come up with our new project during the training. Several reserved projects were chosen and being considered carefully in Spring semester. During the Spring semester, we held project analysis for several times to learn from other teams. ACMA was successfully held as well. General project frame was determined in May with the help of our teachers, advisors, as well as previous iGEMers. Experiments were done from July to October.
     Details of our winter training will also be introduced to you here.
     Winter training was held a few days after Fall semester. In a training lasting for one week, teachers and speakers will present the basic ideas and knowledge related to molecular biology and synthetic biology. Laboratory safety will also be emphasized. Our primary PI Dr. Nianhui Zhang will lead students to do some basic experiments. Timetable of this year’s winter training is shown in figure 1. Students in winter training mainly are reserved team member selected before. The training is not obligated to reserved team members. Though our materials are not available to all students in our college, people who are not in our reserved team but want to participate the training is welcomed. Training material is totally available to them, too. Students will be grouped before the training, including people who are not in our reserved team. Brainstorms were held every night based on groups. On the last day, we will hold project defense to show our primary ideas about project.

      Attributions related to what we mentioned in detail is shown below.

Dr. Nianhui Zhang, team primary PI, teacher of Molecular Biology Experiments in winter training, advisor of project defense, judge of ACMA, provided laboratory for us and pay for expenses on account.
Dr. Chuanfang Wu, team secondary PI, teacher of Molecular Biology in winter training, advisor of project defense, judge of ACMA.

Junyan Zhu, team leader of SCU_China, one of the group leader of melatonin (aimed to produce melatonin successfully and verify enzymes’ function), speaker of Synthetic Biology Discussion in winter training, designed the experiments of group melatonin, expressed four enzymes successfully, wrote related wiki content.
Han Geng, vice team leader of SCU_China, group leader of represillator (aimed to verify the function of represillator), speaker of Synthetic Biology Discussion in winter training, lecturer in CCiC, came up with the original project idea during winter training, measured the fluorescence of repressilator.
Jialu Li, one of the group leader of melatonin, purified four enzymes successfully, also in charge of the reagent purchase this year.
Xinhao Yan, group leader of knockout (aimed to knockout ClpXP enzyme), speaker of Synthetic Biology Discussion in winter training, wrote related wiki content.
Xinpeng Zhang, group leader of modeling, speaker of Synthetic Biology Discussion in winter training, lecturer in CCiC, managed the modeling in our project and give advice based on modeling result, wrote related wiki content.
Yiwen Zhao, group leader of quorum sensing (aimed at synchronizing the oscillated period), designed and constructed quorum sensing circuit, helped in fluorescent measurement of repressilator, wrote related wiki content.
Tianying Chen, one of the group leader of InterLab (aimed to complete InterLab measurement and modified RBS), speaker of Synthetic Biology Discussion in winter training.
Bowen Li, manager of verifying enzyme activities, contacted with Dr. Hui Jin to get the permission of using HPLC with autosampler in College of Pharmacy, ran HPLC for verification, wrote related wiki content.
Yi Shan, art designer, designer of team uniform, poster for ACMA and so on.
Hao Yuan, manager of wiki page, uploaded wiki content, wrote related code and did typesetting.
Fuqiang Yang, manager of InterLab measurement and did related experiments, helped in expression of melatonin and verification of enzyme activities, helped in the survey of human practice, wrote related wiki content.
Wei Lin, did experiments related to construction and verification of quorum sensing circuit, wrote related wiki content.
Zhiwei Luo, did experiments related to expression of melatonin, helped in survey in human practice, wrote related part content.
Yibing Tao, did experiments related to construction and verification of quorum sensing circuit, measured fluorescence of repressilator, wrote related wiki content.
Xuetong Qu, did experiments related to construction and verification of quorum sensing circuit, helpd in organization and survey of human practice.
Haoyang Cheng, did experiments related to expression of melatonin, checkout wiki content written by others.
Yuliang Xiang, did experiments related to verification of enzyme activities by HPLC.
Hongjin Lai, did experiments related to construction of quorum sensing circuit, helped in ClpXP knockout.
Chunyi Yan, did experiments related to ClpXP knockout and helped in construction of quorum sensing circuit and human practices.
Ziru Wang, did experiments related to ClpXP knockout and helped in construction and verification of quorum sensing circuit.
Junjie Xu, did experiments related to RBS modification.
Mingyi Lv, did experiments related to RBS modification.

      First of all, we are very grateful to Sichuan University, College of Life Sciences, Wu Yuzhang Honors College and College of Physical Science and Technology for supporting us in the 2017 iGEM competition. In particular, we are deeply thankful to College of Life Sciences, which provides us with the independent laboratories and laboratory funding.
      Secondly, we want to sincerely acknowledge the seniors and teachers of our university, who encouraged and helped us in different aspects.

Winter Training and ACMA

Dr. Xiaoping Wan, advisor of project defense, offered help in holding ACMA, as well as being one of judge of ACMA.
Dr. Ying Tong, teacher of Commonly Used Experimental Methods in Modern Biology and Case Study in winter training, advisor of project defense, judge of ACMA.
Dr. Jinmin Wu, judge of ACMA.
Dr. Fangdong Zou, judge of ACMA.
Dr. Qun Sun, judge of ACMA.

Project and Experiments

Dr. Fei Huang, advisor of project defense. Dr. Xiaofeng Zhu, advisor of project defense, judge of ACMA, generously provided extra shaker for us.
Dr. Dong Yu, teacher in charge of public laboratory in College of Life Science, taught us how to use HPLC, offered some bacterial filters and injectors when they were used up.
Dr. Han Kang, teacher in charge of plate reader (ThermoScientific Varioskan Flash), assisted us with our fluorescence measurement and InterLab work, manipulating the instrument.
Dr. Zhonghan Li, offered plated reader when instrument managed by Dr. Han Kang is unavailable (though data was not used).
Dr. Chong Chen, offered plated reader which can be used to measure throughout the whole night.
Dr. Hui Jin, teacher in College of Pharmacy of SCU, permitted us to use HPLC with autosampler in College of Pharmacy.
Dr. Ye Zhang, interviewee of Human Practice, accepted our interview in human practice.

      Secondly, we sincerely thank to students and seniors of our university, who provided advice to us.

Qian Jiang, master in College of Pharmacy of SCU, generously offered mice’s feces to us.
Wenjiao Xiao, master in College of Pharmacy of SCU, taught us how to use HPLC with autosampler in College of Pharmacy.
Hongbin Yu, team leader of SCU-WestChina, offered help in optimizing project design, assisted with troubleshooting related to enzyme expression.
Zikuan Song, team member of SCU-WestChina, helped in connecting related officer of Chengdu Library to hold an exhibition.
Jiusi Guo team member of SCU-WestChina, helped in connecting usage of plate reader in Dr. Chong Chen’s lab.
Specially, thanks to team “SCU-WestChina” that helped us in many aspects.

      Last but not least, we are deeply grateful to the labs and iGEMers of other schools that have provided us with help.

Thanks to Jianxin Lv and Wu Wang's lab in Wenzhou Medical University that provided us wide type MC4100 strain for our experiments about repressilator.
Thanks to team “Peking” that provided us ΔclpXP MC4100 stain (DHL708) for our experiments about repressilator.
Thanks to team “SiCAU-China” that provided us LUDOX for our InterLab study.
Thanks to team “TMMU-China” that gave us some suggestions for our project determination.